樟树赣柠1号茎段组织培养研究

Study on Tissue Culture Technology of Stem Segment of Cinnamomum camphora ‘Ganning 1’

关键词:  樟树赣柠1号  组织培养  茎段  生长调节剂  萌芽率  生根率

Key words:  Cinnamomum camphora ‘Ganning 1’  tissue culture  stem segment  growth regulator  germination rate  rooting rate

摘要:

本研究拟建立樟树赣柠1号茎段组织培养技术，为其苗木繁殖提供理论依据。实验以樟树赣柠1号枝条为材料，通过植物组织培养技术，研究枝条木质化程度、外植体的采集月份、消毒时间和生长调节剂对樟树赣柠1号茎段组织培养的影响。结果表明：茎段的污染率和褐化率随枝条木质化程度的增加而升高，萌芽率随枝条木质化程度的增加而降低。5月采集樟树赣柠1号一年生半木质化带芽茎段，用质量浓度0.1%的HgCl₂消毒5 min，茎段的萌芽率高,污染率和褐化率低。樟树赣柠1号茎段萌芽最适培养基为Murashige 和Skoog培养基(MS培养基)+1.2 mg/L 6-苄氨基嘌呤(6-BA)+0.1 mg/L 吲哚丁酸(IBA)，萌芽率为66.67%；最适增殖培养基为MS+2.0 mg/L 6-BA+0.1 mg/L IBA，增殖系数为4.06；生根适宜培养基为1/2 MS+2.0 mg/L IBA，生根率达到100%。组培苗生根培养11-14 d，大棚炼苗7-10 d，移栽成活率可达90.06%。上述组织培养技术条件的建立，可有效提高樟树赣柠1号茎段腋芽的萌芽率、组培苗的增殖率和生根率，为樟树赣柠1号的工厂化育苗奠定基础。

Abstract:

The tissue culture technology of stem segment of Cinnamomum camphora ‘Ganning 1’ was established to provide a theoretical basis for its seedling propagation. In this experiment, the branches of C.camphora ‘Ganning 1’ were used as materials to study the effects of lignification degree of branches, collection month of explants, disinfection time and growth regulators on stem tissue culture of C.camphora ‘Ganning 1’by plant tissue culture technology.The results showed that the pollution rate and browning rate of stems increased with the increase of lignification degree of branches, and the germination rate decreased with the increase of lignification degree of branches.In May, the 1-year-old semi-lignified bud stem segments of C.camphora ‘Ganning 1’ were collected and disinfected with mass concentration 0.1% HgCl₂ for 5 min.The germination rate, pollution rate and browning rate of stem segments were low.The most suitable medium for stem segment germination of C.camphora ‘Ganning 1’ was Murashige and Skoog (MS)+1.2 mg/L 6-benzylaminopurine (6-BA)+0.1 mg/L indole-3-butyric acid (IBA), the germination rate was 66.67%.The most suitable medium for multiplication was MS+2.0 mg/L 6-BA+0.1 mg/L IBA, the multiplication coefficient was 4.06.The suitable medium for rooting was 1/2 MS+2.0 mg/L IBA, and the rooting rate reached 100%.After 11-14 days of rooting culture and 7-10 days of seedling training in greenhouse, the transplanting survival rate of tissue culture seedlings could reach 90.06%.The establishment of the above technical conditions for tissue culture can effectively improve the axillary bud germination rate of stem segment of C.camphora ‘Ganning 1’, the increment rate and rooting rate of tissue culture seedlings, and lay the foundation for the factory seedling of C.camphora ‘Ganning 1’.