工厂化循环水养殖珍珠龙胆石斑鱼和红鳍笛鲷肠道菌群的多样性

Diversity in the Gut of Pearl Gentian Grouper (Epinephelus fuscoguttatus ♂×E.lanceolatu ♀) and Crimson Snapper (Lutjanus erythropterus) Cultured in Industrial Recirculating Aquaculture System

关键词:  工厂化循环水系统  珍珠龙胆石斑鱼  红鳍笛鲷  肠道菌群  高通量测序

Key words:  recirculating aquaculture system  Epinephelus fuscoguttatus ♂×E.lanceolatu ♀  Lutjanus erythropterus  bacterial community  high-throughput sequencing

摘要:

鱼类肠道菌群的组成与宿主健康紧密相关，且受众多因素的影响。为分析工厂化循环水养殖珍珠龙胆石斑鱼(Epinephelus fuscoguttatus ♂×E.lanceolatu ♀)和红鳍笛鲷(Lutjanus erythropterus)肠道细菌组成及其与养殖环境菌群的关系，本研究采用高通量测序的方法对两种鱼肠道和养殖水体的细菌组成及多样性进行分析。结果表明珍珠龙胆石斑鱼和红鳍笛鲷肠道细菌与其各自养殖水体中共有的可操作分类单元(Operational Taxonomic Unit，OTU)占比分别为19.39%和25.68%,珍珠龙胆石斑鱼中有高达75.41%的OTUs与红鳍笛鲷一致。两种鱼肠道微生物之间的相似性比其养殖水体中的细菌相似性高。珍珠龙胆石斑鱼肠道优势菌群为厚壁菌门Firmicutes、变形菌门Proteobacteria中的α-变形菌纲Alphaproteobacteria和拟杆菌门Bacteroidetes，红鳍笛鲷肠道优势菌群为变形菌门中的δ-变形菌纲Deltaproteobacteria和γ-变形菌纲Gammaproteobacteria。综上所述，珍珠龙胆石斑鱼和红鳍笛鲷肠道细菌均独立于其各自养殖海水细菌群落；在同一个工厂化循环水系统养殖两种鱼类，其肠道细菌种类大部分一致，但关键的优势菌群并不同。

Abstract:

The composition of fish intestinal flora is closely related to host health, and is affected by many factors.In order to analyze the composition of intestinal bacteria between pearl gentian grouper (Epinephelus fuscoguttatus ♂×E.lanceolatu ♀) and crimson snapper (Lutjanus erythropterus) reared in an Recirculating Aquaculture System (RAS) and their relationship with the flora in the culture environment, the bacterial composition and diversity of microflora in intestine and cultured water were analyzed by high-throughput sequencing.The results show that the proportion of common OTUs between the intestinal bacteria of pearl gentian grouper and crimson snapper with their cultured water is 19.39% and 25.68% respectively.As high as 75.41% of OTUs in pearl gentian grouper are consistent with those in crimson snapper.The similarity in bacterial community between the guts of two fish species is higher than that of bacteria in aquaculture water.The dominant intestinal flora of pearl gentian grouper are Firmicutes, Alphaproteobacteria and Bacteroidetes, while the dominant intestinal flora of crimson snapper are Deltaproteobacteria and Gammaproteobacteria.To sum up, the gut bacteria of pearl gentian grouper and redfin snapper are independent of their cultured seawater bacterial communities.The gut bacteria of two fish species cultured in the same industrial recirculating water system are mostly the same, but the key dominant bacteria are different.