两种养殖模式下红文蛤的代谢组差异分析

Study on Metabolic Differences of Red Meretrix meretrix under Two Culture Modes

关键词:  养殖模式  红文蛤  LC-MS  代谢组  多元统计  差异代谢物  差异代谢通路  氨基酸

Key words:  culture modes  red Meretrix meretrix  LC-MS  metabolome  multivariate statistics  differential metabolites  differential metabolic pathway  amino acid

摘要:

为探究不同养殖模式下红壳色文蛤(以下简称“红文蛤”，red Meretrix meretrix)的代谢差异，以单养池塘(D组)和虾蛤混养池塘(H组)的红文蛤为研究对象，通过实验生态学方法和液相色谱-质谱联用仪(LC-MS)技术对其进行代谢组学分析。结果显示，在不同养殖模式下共筛选出151种差异代谢物，京都基因及基因组百科全书(KEGG)通路注释富集到9条代谢通路(P<0.05，IP>0.2)，这些通路与氨基酸及其衍生物的代谢合成有关，其中涉及多条代谢通路的谷氨酸、天冬氨酸、谷氨酰胺是关键差异代谢物。氨基酸验证结果表明，两种养殖模式下的氨基酸含量存在差异，这与代谢组的研究结果基本一致。虾蛤混养模式下红文蛤氨基酸代谢水平优于单养池塘，不同养殖模式可能通过影响红文蛤的氨基酸代谢，进而影响其免疫及抗氧化能力。上述结果可为红文蛤在不同养殖模式下的代谢响应机制研究和科学健康养殖及养殖模式的构建提供理论参考。

Abstract:

In order to explore the metabolic differences of red Meretrix meretrix under different culture modes,the red M.meretrix in monoculture ponds (group D) and shrimp-clam polyculture ponds (group H) were taken as the research objects,the metabolomics analysis was carried out by experimental ecological methods and Liquid Chromatography Mass Spectrometry (LC-MS) technology.The results showed that a total of 151 differential metabolites were selected under different culture modes,and 9 metabolic pathways (P<0.05，IP>0.2) were obtained by Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway annotation.These pathways were related to the metabolic synthesis of amino acids and their derivatives,among which glutamic acid,aspartic acid and glutamine involved in multiple metabolic pathways were the key differential metabolites.The results of amino acid verification showed that there were differences in amino acid content between the two culture modes,which were basically consistent with the results of metabolomics study.The amino acid metabolism level of M.meretrix in polyculture mode is better than that in monoculture pond.Different culture modes may affect the immune and antioxidant capacity of red M.meretrix by affecting the amino acid metabolism.The above results can provide theoretical reference for the study of metabolic response mechanism of M.meretrix under different culture modes and the construction of scientific and healthy culture and culture mode.