| 引用本文： |
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李玉芝,宋肖敏婷,裴瑾,曹治兴,陈江,饶华楠.响应面法优化南方红豆杉中10-DAB提取工艺及其含量测定[J].广西科学院学报,2023,39(3):314-321. [点击复制]
- LI Yuzhi,SONG Xiaominting,PEI Jin,CAO Zhixing,CHEN Jiang,RAO Hua'nan.Response Surface Methodology for Optimization of Extraction Process and Content Determination of 10-DAB in Taxus wallichiana var.mairei[J].Journal of Guangxi Academy of Sciences,2023,39(3):314-321. [点击复制]
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| 摘要: |
| 为探究南方红豆杉（Taxus wallichiana var.mairei）中10-去乙酰基巴卡亭Ⅲ（10-DAB）的最佳提取工艺，本研究以南方红豆杉中10-DAB含量为指标，以料液比、提取时间及超声温度为考察因素，采用单因素试验结合响应面法优化10-DAB的提取工艺，并采用超高效液相色谱（UPLC）法检测不同采摘部位、不同生长年限及不同采收时节的10-DAB含量差异。结果表明，南方红豆杉中10-DAB的最佳提取工艺条件为料液比1∶15（g/mL）、提取时间23 min、超声温度40℃，该工艺条件下10-DAB的含量为1.18%，与理论值相对偏差为0.42%。UPLC检测结果表明，南方红豆杉叶的10-DAB含量显著高于枝和树皮，且10-DAB全年累积含量受季节影响明显，10月份达到最高值。该研究可为南方红豆杉中10-DAB的提取利用提供参考依据。 |
| 关键词: 南方红豆杉 10-DAB 响应面分析 单因素试验 超高效液相色谱法 |
| DOI：10.13657/j.cnki.gxkxyxb.20230829.011 |
| 投稿时间：2023-02-19修订日期：2023-03-30 |
| 基金项目:四川省科技厅项目（18ZDZX0075）资助。 |
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| Response Surface Methodology for Optimization of Extraction Process and Content Determination of 10-DAB in Taxus wallichiana var.mairei |
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LI Yuzhi, SONG Xiaominting, PEI Jin, CAO Zhixing, CHEN Jiang, RAO Hua'nan
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| (State Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, 611137, China) |
| Abstract: |
| In order to explore the optimal extraction process of 10-Deacetylbaccatin Ⅲ (10-DAB) from Taxus wallichiana var.mairei leaves, the content of 10-DAB in T.wallichiana var.mairei was taken as the index, and the solid-liquid ratio, extraction time and ultrasonic temperature were taken as the investigation factors.The extraction process of 10-DAB was optimized by single factor test combined with response surface method, and the content difference of 10-DAB in different picking parts, different growth years and different harvesting times was detected by Ultra-high Performance Liquid Chromatography (UPLC). The results showed that the optimum extraction conditions of 10-DAB from T.wallichiana var.mairei were as follows:the solid-liquid ratio was 1:15 (g/mL), the extraction time was 23 min, and the ultrasonic temperature was 40℃.Under these conditions, the content of 10-DAB was 1.18%, and the relative deviation from the theoretical value was 0.42%.The results of UPLC detection showed that the content of 10-DAB in the leaves of T.wallichiana var.mairei was significantly higher than that in branches and barks, and the annual cumulative content of 10-DAB was obviously affected by the season, reaching the highest value in October.This study can provide a reference for the extraction and utilization of 10-DAB in T.wallichiana var.mairei. |
| Key words: Taxus wallichiana var.mairei 10-DAB response surface methodology single factor experiment UPLC method |
