3种苏铁属植物总黄酮、总多糖含量及抗氧化活性研究

Study on the Content of Total Flavonoids and Total Polysaccharides and Antioxidant Activity in Three Species of Cycas Plants

关键词:  锈毛苏铁  叉孢苏铁  石山苏铁  黄酮  多糖  抗氧化活性

Key words:  Cycas ferruginea  Cycas segmentifida  Cycas sexseminifera  flavonoids  polysaccharides  antioxidant activity

摘要:

为探究锈毛苏铁(Cycas ferruginea)、叉孢苏铁(C.segmentifida)和石山苏铁(C.sexseminifera) 3种苏铁属(Cycas)植物的总黄酮、总多糖含量以及抗氧化活性，本研究采用超声波辅助法对其根、叶柄、叶、雄球花、茎干的总黄酮及总多糖含量进行提取，以DPPH自由基(DPPH·)、羟自由基(·OH)、超氧阴离子自由基(O^-₂·)清除率评价其抗氧化活性,并采用Pearson相关性分析法分析相关性。结果表明：锈毛苏铁、叉孢苏铁和石山苏铁叶的总黄酮含量较高，大小依次为锈毛苏铁(8.61 mg·100 mg^-1)>叉孢苏铁(7.82 mg·100 mg^-1)>石山苏铁(1.04 mg·100 mg^-1)；茎干中总多糖含量最高,大小依次为叉孢苏铁(28.32 mg·100 mg^-1)>石山苏铁(24.43 mg·100 mg^-1)>锈毛苏铁(16.59 mg·100 mg^-1)。锈毛苏铁与叉孢苏铁叶对DPPH·的清除能力较好,3种苏铁属植物各个部位对·OH的清除率能力均较差，叉孢苏铁根对O^-₂·的清除能力较好。综上，3种苏铁属植物各部位抗氧化活性主要与黄酮含量有关，该研究可为广西苏铁属植物的综合利用提供科学依据。

Abstract:

In order to investigate the contents of total flavonoids,and total polysaccharides and antioxidant activities in three species of Cycas plants,C.ferruginea,C.segmentifida and C.sexseminifera,the contents of total flavonoids and total polysaccharides from their roots,petioles,leaves,male flowers and stems were extracted by ultrasonic-assisted method,and their antioxidant activities were evaluated by DPPH free radical (DPPH·),hydroxyl radical (·OH) and superoxide anion radical (O^-₂·) scavenging rates.Pearson correlation analysis was used to analyze correlation.The results showed that the contents of total flavonoids in the leaves of C.ferruginea,C.segmentifida and C.sexseminifera were relatively higher,and the order was C.ferruginea (8.61 mg·100 mg^-1)>C.segmentifida (7.82 mg·100 mg^-1)>C.sexseminifera (1.04 mg·100 mg^-1).The content of total polysaccharides in the stems was the highest,and the order was C.segmentifida (28.32 mg·100 mg^-1)>C.sexseminifera (24.43 mg·100 mg^-1)>C.ferruginea (16.59 mg·100 mg^-1).The scavenging ability of DPPH· was better in the leaves of C.ferruginea and C.segmentifida.The scavenging ability of ·OH was poor in all parts of the three species of Cycas plants,and the scavenging ability of O^-₂· was better in the roots of C.segmentifida.In summary,the antioxidant activity of each part of the three Cycas plants is mainly related to the content of flavonoids.This study can provide a scientific basis for the comprehensive utilization of Cycas plants in Guangxi.