基于RNA-seq测序的弓獭蛤(Lutraria rhynchaena)不同组织转录组差异研究

Study on Transcriptome Differences in Different Tissues of Lutraria rhynchaena Based on RNA-seq Sequencing

关键词:  弓獭蛤  组织  高通量测序  转录组  生物信息学分析

Key words:  Lutraria rhynchaena  tissues  high throughput sequencing  transcriptome  bioinformatics analysis

摘要:

弓獭蛤(Lutraria rhynchaena)是我国南方沿海重要的海水养殖贝类，为探讨弓獭蛤不同组织的基因表达差异，本研究采用RNA-seq测序技术对北部湾海域弓獭蛤7种组织(肌肉、外套膜、鳃、肝胰腺、虹吸管、雌性性腺和雄性性腺)进行转录组测序及生物信息学分析。结果显示，21个样本共获得939 197 518条质控后序列，各组织质控后的碱基数为15.39-29.26 G。测序结果经过Denovo组装和ORF查找共获得56 773个Unigenes。在5个公共数据库NR (Non-Redundant protein sequence database)、GO (Gene Ontology)、eggNOG(evolutionary genealogy of genes:Non-supervised Orthologous Groups)、KEGG(Kyoto Encyclopedia of Genes and Genomes)和SWISS-Prot对比各注释分别得到24 557、13 094、17 524、10 352和13 857条有效注释信息。本研究获得了大量弓獭蛤组织间的差异表达基因，丰富了弓獭蛤的基因数据库，为更深入地了解弓獭蛤各组织功能，进一步挖掘和开发利用弓獭蛤功能基因提供基础数据。

Abstract:

Lutraria rhynchaena is an important mariculture shellfish in the southern coast of China.In order to explore the differences in gene expression in different tissues of L.rhynchaena,RNA-seq sequencing technology was used to perform transcriptome sequencing and bioinformatics analysis on seven tissues (muscles,mantles,gills,hepatopancreas,siphons,female gonads and male gonads) of L.rhynchaena in the Beibu Gulf.The results showed that a total of 939 197 518 sequences after quality control were obtained from 21 samples,and the number of bases in each tissue was 15.39-29.26 G after quality control.A total of 56 773 Unigenes were obtained by Denovo assembly and ORF search.A total of 24 557,13 094,17 524,10 352 and 13 857 valid annotations were obtained by comparing each annotation in five public databases NR (Non-Redundant protein sequence database)、GO (Gene Ontology)、eggNOG (evolutionary genealogy of genes:Non-supervised Orthologous Groups)、KEGG (Kyoto Encyclopedia of Genes and Genomes) and SWISS-Prot.In this study,a large number of differentially expressed genes between tissues of L.rhynchaena were obtained,and the gene database of L.rhynchaena was enriched,which provided basic data for a deeper understanding of the functions of various tissues of L.rhynchaena,and further exploration and development of functional genes of L.rhynchaena.