海洋卡盾藻及其化感物质对链状裸甲藻的的氧化胁迫研究

oxidative stress of Chattonella marina and its allelopathic on Gymnodinium catenatum

关键词:    :藻链状裸甲藻  海洋卡盾藻  化感作用  棕榈油酸  氧化损伤

Key words:  Gymnodinium catenatum  Chattonella marina  Allelopathic  Palmitoleic acid  Oxidative damage

摘要:

摘要：【目的】链状裸甲藻是我国东南沿海的常见有毒赤潮原因种，其产生的麻痹性贝类毒素给渔业和水产养殖业带来了巨大的经济损失，且该问题在全球范围内日益严重。有研究表明，海洋卡盾藻对链状裸甲藻有明显的化感抑制作用，但是对二者之间化感作用的机制鲜有研究。【方法】通过研究海洋卡盾藻对链状裸甲藻的化感作用，并研究不同棕榈油酸浓度下氧化胁迫诱导链状裸甲藻细胞死亡的现象，揭示了海洋卡盾藻的化感物质对链状裸甲藻的抑藻机制。【结果】本研究发现海洋卡盾藻中的棕榈油酸能够显著的抑制链状裸甲藻的生长，抑制效果随着棕榈油酸浓度的增大而增强，且棕榈油酸能够对链状裸甲藻的抗氧化系统造成影响，导致链状裸甲藻细胞内O2 ·-的过度积累而引起膜脂过氧化，Caspase-3含量也随着棕榈油酸浓度的增加呈现上升趋势。【结论】因此认为棕榈油酸造成了链状裸甲藻的过氧化损伤从而导致了链状裸甲藻细胞的凋亡为化感作用的机理。

Abstract:

Abstract:【Objective】 Gymnodinium catenatum is a common cause of toxic red tide along the southeast coast of China, and the paralytic shellfish toxins produced by it have brought huge economic losses to the fisheries and aquaculture industry, and the problem is becoming more and more serious worldwide. Studies have shown that Chattonella marina has a significant allelopathic inhibitory effect on Euglenalis streptoiformis, but the mechanism of allelopathic interaction between the two has been seldom studied.【Methods】To study the allelopathy of Chattonella marina. on Gymnodinium catenatum, and to study the phenomenon of cell death induced by oxidative stress at different palmitoleic acid concentrations, the inhibition mechanism of allelochemicals from Chattonella marina on Gymnodinium catenatum was revealed.【Results】In this study, it was found that the palmitoleic acid in Chattonella marina dinoflagellates could significantly inhibit the growth of Gymnodinium catenatum, and the inhibitory effect increased with the increase of palmitoleic acid concentration, and palmitoleic acid could affect the antioxidant system of Gymnodinium catenatum, resulting in the excessive accumulation of O2·- in the cells of Gymnodinium catenatum and causing membrane lipid peroxidation, and the content of Caspase-3 also showed an upward trend with the increase of palmitoleic acid concentration.【Conclusion】Therefore, it is believed that palmitoleic acid causes peroxidative damage of Gymnodinium catenatum, which leads to the apoptosis of Gymnodinium catenatum , which is the mechanism of allelopathy. Key words：Gymnodinium catenatum ;Chattonella marina ;Allelopathic ; Palmitoleic acid; Oxidative damage