巴西铁的茎组织培养

Stem Culture in Vitro of Dracaena sanderiena

关键词:  巴西铁  茎离体培养  再生植株

Key words:  Dracaena sanderiena  stem culture in vitro  regrowth plant

摘要:

以巴西铁(Dracaena sanderiena)茎段为接种材料,MS为基本培养基,吲哚乙酸(IAA)、茶乙酸(NAA),2,4-二氯苯氧乙酸(2.4-D)、6-苄氨基嘌呤(6-BA)、6一糠基氨基嘌呤(6-FA)、吲哚丁酸(IBA)、生根粉(ABT₁)、玉米素(ZT)为附加成分,进行诱导、分化、生根试验。诱导结果表明:IAA不起作用,2,4-D有较好的促进作用,0.5mg/L(2,4-D)+2mg/L(6-BA)组合,诱导率高达90%。分化结果表明:NAA、6-BA效果较好,6-FA作用不明显,lmg/LNAA+4ms/L(6-BA)+2ms/LZT组合,分化率最高.达81.1%。生根结果表明:MS+0.5mg/LNAA+0.5mg/L(6-FA),MS+0.4mg/LNAA+2mg/LIBA,MS+0.2mg/LNAA+0.1mg/L(6-FA)+ABT₁三种培养基均发根,生根率70%以上,根粗,但有点脆,若培养基中加入活性碳产生的根则细而韧。

Abstract:

Stem sections of Dracaena sanderiena as inoculative material, MS as basic culture medium,indoleacetic acid (IAA), naphthyl acetic acid (NAA), 2, 4-dichlorophenoxyacetic acid (2, 4-D), 6-benzylaminopurine (6-BA), 6-furfurylaminopurine (6-FA), indolebutyric acid (IBA), ABT1 (commercial name) and zeatin (ZT) as additional compositions were used for inducing, differentiating and rooting tests. Induced results showed that 2, 4-D had better promoting effect; the combination of 0.5mg/L (2, 4-D) and 2 mg/L (6-BA) had 90 percent of induction, but IAA had no effect. NAA and 6-BA showed a better differentiation effect; the combination of 1 mg/L NAA+ 4 mg/L (6-BA) 42 mg/L ZT had a high differentiation percentage, up to 81.1%, but 6-FA had not apparent effect on differentiation. The rooted results showed that these three culture media, MS+0.5 mg/L NAA+0.5.mg/L (6-FA), MS+0.4 mg/L NAA+2 mg/L IBA, MS+0.2 mg/L NAA+0.1 mg/L (6-FA) +ABT1, all emerged roots, the rooted ratio were 70% above. The roots showed thick as if active carbon was added to the culture medium, the roots emerged were thin and tenacious.