水牛血清乳酸脱氢酶同工酶与产奶量的关系

A Study on the Relation between the Serum LDH Isozymes and the Milk Output in Buffalo Cows

关键词:  水牛  血清乳酸脱氢酶  同工酶谱型  产奶量

Key words:  buffalo  serum lactate dehydrogenase (LDH)  electrophorograph of isozyme  milk output

摘要:

用聚丙烯酰胺凝胶圆盘电泳和光密度扫描法,对摩拉(Murrah)、尼里(Nili-Ravi)及三品系杂交[Ni♂X(Mu♂×Nanning♀)♀]三种母奶水牛血清LDH同工酶谱型、活力及其与产奶量的关系进行了定量测定和"分析。再次确证其同工酶谱型特点是LDH₁ > LDH₂ > LDH₃ > LDH₄ > LDH₅和LDH₆的发现。其相对总活力为尼里 > 摩拉 > 三品杂。种间LDH₁和LDH₂的相对百分含量与产奶量呈正相关趋势,LDH₃与之正相反,LDH₄和LDH₅是尼里 < 摩拉 < 三品杂。种内其相对总活力和相对百分含量在LDH₁的三种牛、LDH₂的尼里和三品杂、LDH₅的摩拉等均与产奶量呈正相关趋势,而在LDH₂的摩拉、LDH₃、LDH₄、LDH₅的尼里和三品杂等则与之正相反。

Abstract:

The electrophorographs, the activity of serum LDH isozymes and the relation between the milk output and the enzymic activity of serum LDH isozymes in three breeds of buffalo cows,Murrah, Nili-Ravi and a Hybrid (Hy)[Ni♂×(Mu♂×Nanning ♀)♀], were quantitively measured and analyzed. The electrophorographs were further demonstrated to be LDH₁ > LDH₂ > LDH₃ > LDH₄ > LDH₅. A new band of LDH₆ was discovered. The relative total activity is Ni > Mu > Hy.The relative percentages of both LDH₁ and LDH₂ were positively correlated to the milk output, and the opposite was true for LDH₃, LDH₄ and LDH₅ are Ni < Mu < Hy. The relative total activity and relative percentages of LDH₁ in the three breeds, of LDH₂ in Ni and Hy and of LDH₅ in Mu were correlated to the milk output, and the opposite was true for LDH₂ in Mu and LDH₃, LDH₄ and LDH₅ in Ni and Hy.