| 引用本文： |
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张红星,吴宝成,徐振东,唐铃弟.鸡新城疫水-油-水型活性复乳的研制和免疫研究[J].广西科学,1996,3(3):45-47. [点击复制]
- Zhang Hongxing,Wu Baocheng,Xu Zhendong,Tang Lingdi.Preparation and Immune Effect of W/O/W Active Multiple Emulsion against Newcastle Disease Virus[J].Guangxi Sciences,1996,3(3):45-47. [点击复制]
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| 摘要: |
| 水-油-水(W/O/W)型活性复乳是将油包水初乳(W/O)进一步分散在水相中经过二次乳化,使用时并配以新城疫弱毒疫苗联合制成。采用透析法测定复乳的形成率分别为95.0%,89.3%,88.0%和84.4%,雏鸡免疫后4dHI抗体即明显升高,井持续2个月以上;对育成鸡的加强免疫持续期达14个月,滴鼻或肌注攻毒全保护,证实新城疫W/O/W活性复乳已达到实用要求。 |
| 关键词: 新城疫 活性复乳 免疫 |
| DOI： |
| 投稿时间：1996-02-26修订日期：1996-05-20 |
| 基金项目: |
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| Preparation and Immune Effect of W/O/W Active Multiple Emulsion against Newcastle Disease Virus |
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Zhang Hongxing1, Wu Baocheng1, Xu Zhendong1, Tang Lingdi2
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| (1.Dept. of Animal Husbandry and Veterinary Medicine, Guangxi Argricultural University, 13 Xiuling Road, Nanning, Guangxi, 530005;2.Guangxi Academy of Sciences, 20 Xiyili, Jiangnan Road, Nanning, Guangxi, 530031) |
| Abstract: |
| Newcastle disease (ND) is a serious infectious disease for the poultry industry in China,and occurres frequently though immunization of active and inactive vaccine against ND has been widespread applied. In this paper, W/O/W active multiple emulsion (multiemulsion) has been prepared at first by way of second emulsifying in exophase of water with primary oil emulsion (W/O) of inactive vaccine against ND, and active vaccine is added when immunization will be carried out.The results show:the forming percentage of the multiple emulsion are respectively 95.0, 89.3,88.0 and 84.4 using Permeability's method. HI antibody titers against NDV raise obviously after immuned 4 day and maintain up to 2 months for young chickens, and the immune effect could maintain up to 14 months after reinforcing immune for three-month-old chickens. The protected rate of immuned chickens using W/O/W active multiple emulsion are all 100% for challenge of NDV. |
| Key words: newcastle disease active multiemulsion immunity |
