| 引用本文： |
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马韵,邓卓霖,罗虹,丁志敏.黄曲霉毒素高污染区肝细胞癌P53基因高频率定点突变[J].广西科学,1996,3(3):48-50. [点击复制]
- Ma Yun,Deng Zhuolin,Luo Hong,Ding Zhimin.High Frequency of P53 Gene Fixed Point Mutation in Hepatocellular Carcinoma from AFB1 High Risk Area[J].Guangxi Sciences,1996,3(3):48-50. [点击复制]
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| 摘要: |
| 用聚合酶链反应及限制性片段长度多态性(PCR-RFLP)分析技术,对广西黄曲霉毒素高污染区52例肝细胞癌(HCC)进行分析,检查P53基因第7外显子的249密码子的突变频率.结果发现36/52例HCC中249密码子有集中的点突变,频率为69.2%。P53基因突变热点与乙型肝炎病毒感染无关。提示黄曲霉毒素B1是突变热点的主要原因。 |
| 关键词: 肝细胞癌 P53基因 突变热点 乙型肝炎病毒 黄曲霉毒素B1 |
| DOI： |
| 投稿时间：1996-06-10 |
| 基金项目:国家自然科学基金 |
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| High Frequency of P53 Gene Fixed Point Mutation in Hepatocellular Carcinoma from AFB1 High Risk Area |
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Ma Yun, Deng Zhuolin, Luo Hong, Ding Zhimin
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| (Dept. of Pathology, Guangxi Medical Univ., 6 Binhu Road, Nanning, Guangxi, 530021) |
| Abstract: |
| Fifty two cases of hepatocellular carcinoma (HCC) from high aflatoxin B1 (AFB1) contaminated area in Guangxi were studied by polymerase chain reaction and restrictive fragment length polymorphism (PCR-RFLP) analysis to examine the exon 7 of P53 gene in HCC. Mutation points were mostly clustered at codon 249 to form a mutational hot-spot. The frequency was 36/52 (69.2%). It seems that there has no relation to hepatitis B virus infection. The result indicates that AFB1 is the most important agent for the specific mutation at hot-spot in the P53 gene of human hepatocellular carcinoma. |
| Key words: hepatocellular carcinoma P53 gene mutational hot-spot hepatitis B virus aflatoxin B1 |
