哮喘患者肺泡巨噬细胞中IL-6mRNA表达的研究

Investigation of Interleukin-6 mRNA Expression in Asthmatic Alveolar Macrophages

关键词:  白细胞介素-6  基因表达  巨噬细胞  哮喘

Key words:  interleukin-6  gene expression  alveolar macrophage  asthma

摘要:

应用逆转录酶链反应及索氏转移杂交技术分析过敏性支气管哮喘患者(激素治疗组9例,非激素治疗组12例)及正常对照组(10例)肺泡巨噬细胞(alveolarmacrophages,AM)中白细胞介素一6(interleukin-6,IL-6)的mRNA表达。IL-6/β-actin比值表示。结果发现未用激素治疗的哮喘患者AM的IL-6/β-actin为0.66±0.32,显著高于正常对照者(0.34±0.13,P<0.01)及激素治疗组(0.41±0.15,P<0.05)。提示表达IL-6的AM在哮喘的发病机理中可能具有重要的作用,而皮质激素治疗哮喘的机制之一可能与其抑制AM表达及产生IL-6的能力有关。

Abstract:

The interleukin-6 (IL-6) mRNA expression was analyzed in alveolar macrophage (AM) from allergic asthmatics treated with corticosteriod (9 cases) or not (12 cases), as well as normal subjects (10 cases). This was achieved by using reverse transcription-polymerase chain reaction and Southern blots techniques. The result was described by IL-6/β-actin ratio and was found that the x ±s of IL-6/β-actin ratio in AM from asthmatics without corticosteriod treatment was 0.66±0.32, which significantly higher than that from either normal subjects (0.34±0.13, P<0.01) or asthmatics treated with corticosteried (0.41±0.15, P<0.05). These results suggest that AM expression of IL-6 may be important in phathogenesis of human asthma, and that one respect of mechanisms of corticosteriod in asthma treatment may be inhibition of IL-6 and thus supression of inflammation in airway.