大鼠肝癌变的启动、促进、发展和癌形成多阶段中癌基因原位表达的动态观察

In Situ Expression of Oncogenesis during Multiple Stages in Hepatocarcinogenesis in Rats

关键词:  实验性肝细胞癌  原位杂交  c-myc  N-ras  动物实验

Key words:  experimental hepatocellular carcinoma  in situ hybridization  c-myc  N-ras  animal experiment

摘要:

用原位分子杂交方法动态观察Solt-Farber模型实验大鼠肝癌发生的启动、促进、发展,癌形成各阶段c-myc,N-ras的表达及其与病理改变及γ-GT酶表达的关系。结果,这两种癌基因在促癌阶段出现高表达,继而略下降,诱癌中期以后随增生结节的发展和癌的形成呈增加的趋势,与γ-GT酶的表达有一定的吻合性。诱癌中晚期c-myc,N-ras的表达增高与癌变关系密切。结果表明,c-myc和N-ras在肝癌的发生中有协同作用,从分子水平进一步证实肝细胞增生结节与肝细胞癌变关系密切。

Abstract:

The relationship among the expression of c-myc,N-ras,histologic alterations and the expression of γ-GT was investigated during the stages of initiation,promotion,development and neoplasia of chemical hepatocarcinogenesis in rats using in situ hybridization and histochemistry. Hepatocarcinogenesis was induced according to the model of Solt and Farber.The results showed that the expression of these two oncogenesis were increased started at promotion stage,follow a little decreasing, then increased again.From the middle stage of hepatocarcinogenesis, the expression of c-myc,N-ras was increased and correlated with the expression of γ-GT,the progression of neoplastic nodules and the formation of hepatocellular carcinoma. A close relation between increased expression of c-myc,N-ras and the was found in middle and late stages of hepatocarcinogenesis.The present data suggest that there might be a synergism between c-myc and N-ras in hepatocarcinogenesis.It is further confirmed that there is a close correlation between hepatocytic nodules and the by molecular biologic technique.