耐长春新碱人卵巢癌细胞系的建立和耐药机制的研究

Establishment of a New Vincristine-resistant Human Oophoroma Cell Line and its Mechanism of Drug Resistance

关键词:  卵巢肿瘤  长春新碱  药物耐受性  细胞系

Key words:  oophoroma  vincristine  multidrug resistance  cell line

摘要:

采用浓度递增和短期作用法,从人卵巢上皮癌细胞A2780中筛选培养出对长春新碱(VCR)耐药的细胞亚株(A2780/VCR),并对其耐药指数、交叉耐药性,细胞内VCR含量以及细胞多药耐药基因,谷胱甘肽S转移酶表达进行检测,结果:A2780/VCR对VCR的耐药指数高达61000倍,并呈多药耐药性;A2780经VCR诱导耐药后,细胞内VCR含量为0;耐药细胞内MDR1表达呈阳性,而GST-π表达则为阴性。上述结果表明A2780/VCR为一获得性高耐受性细胞系,并呈多药耐药特征,其耐药机理与多药耐药基因表达有关。

Abstract:

A novel VCR-resistant cell line was developed by gradually increasing dose and shortly acting with VCR and its tolerance,cross tolerance, VCR content of cell and the multidrug resistance gene (MDR₁) of cell and glutathion transferase-π(GST-π) was measured.A2780/VCR cell was 61000-fold to VCR and released characterization of multidrug resistance.Compared with A2780,the content of VCR in A2780/VCR is zero. In A2780/VCR, positive MDR 1 and negative GST-π. These results showed that A2780/VCR cell line was acquired resistance drug with a characterization of multidrug resistance,and the A2780/VCR resistance to VCR was correlated with overexpression of MDR₁.