马氏珠母贝与解氏珠母贝的随机扩增多态DNA分析

Comparative Analysis on Random Amplified Polymorphic DNA between Pinctada martensii (Dunker) and P.chemmitri Philippi

关键词:  马氏珠母贝  解氏珠母贝  RAPD分析  遗传多样性

Key words:  Pinctada martensii(Dunker)  P chemmitri Philippi  random amplified polymorphic DNA (RAPD)  genetic diversity

摘要:

用OPM组20种碱基随机引物对采自海南洋浦的马氏珠母贝(Pinctada martensii)和解氏珠母贝(P.chemmitri)天然群体进行RAPD分析,其中6种引物扩增有效。这6种引物对马氏珠母贝和解氏珠母贝的总扩增带数分别为52条和58条。每一引物的扩增带数为3~16条,同一引物对两种贝类的扩增带数不同(OPM19除外),对两贝类的扩增带型也有较大差异。群体内的各个个体(实验个体为10个)RAPD扩增带谱全部呈多态,表明在同一群体中,各个体存在着明显的个体特征带。由于样品数量及引物数均少,2种贝类各自种群的共同特征尚未能确定。

Abstract:

The 20 random primers of Group M made in Operon Company were applied to the random amplified polymorphic DNA (RAPD) analysis on natural individuals of Pinctada martensii (Dunker) and P. chemmitri Philippi from Yangpu Harbor, Hainan Province, southern China. Fifty two and fifty eight bands were amplified successfully by six of 20 primers to P.martensii and P.chemmitri respectively. Each primer amplified three to sixteen bands. The number of bands amplified by the same primer (except for OPM 19 primer) is different in the two species, and their type of bands is clearly different. All bands from individuals (ten individuals in the trial) of a species are diverse. It is suggested that each individual in different species has its own special bands. The species characteristic could not be determined owing to less samples and primers.