用GC/MS、GC分析中药前胡中白花前胡丙素、丁素和紫花前胡苷元

Analysis of dl-Praeruptorin A,dl-Praeruptorin B and Nodakenetin in Qianhu by GC/MS and GC

关键词:  前胡  白花前胡丙素  白花前胡丁素  紫花前胡苷元  气相色谱/质谱

Key words:  Peucedanum  dl-praeruptorin A (Pd-Ⅰa)  dl-praeruptorin B (Pd-Ⅱ)  Nodakenetin  gas chromatography/mass spectrometry

摘要:

为快速鉴别中药白花前胡(Peucedanum praeruptorum Dunn.)和紫花前胡(Peucedanum decursivum Maxim.),用气相色谱/质谱(GC/MS)、气相色谱法(GC)分别定性、定量分析白花前胡丙素(Pd-Ia)、丁素(Pd-Ⅱ))和紫花前胡苷元(Nodakenetin)。在白花前胡中检出白花前胡丙素(M/Z386)和丁素(M/Z426),但未检出紫花前胡苷元;在紫花前胡中检出紫花前胡苷元(M/Z246),但未检出白花前胡丙素和丁素。线性关系:Y_Pd-Ia=1.111X_Pd-Ia+0.021(n=6 r=0.9995),线性范围:0.175~1.919μg。Y_Pd-Ⅱ=1.189X_Pd-Ⅱ-0.013(n=6 r=0.9996),线性范围:0.086~0.950 μg。Y_Nodakenetin=0.90 1X_Nodakenetin-0.024(n=6 r=0.9999),线性范围:0.089~1.062μg。回收率:白花前胡丙素97.29%,白花前胡丁素98.81%,紫花前胡苷元96.34%。分析结果表明,不同产地药材样品中各自特征成分含量不同,分析中未能找到白花前胡和紫花前胡苷元共有的相同的有效成分或指标性成分。表明可用GC/MS区分白花前胡和紫花前胡苷元,但未能用统一的化学对照品进行质量控制。

Abstract:

For quick distinguishing Peucedanum praeruptorum Dunn and Peucedanum decursivum Maxim,the gas chromatography/mass spectrometry (GS/MS) and gas chromatography (GS) are used to analyze dl-praeruptorin A(Pd-Ⅰa),dl-praeruptorin B(Pd -Ⅱ) and Nodakenetin in the root samples of Qianhu (Peucedanum).The Pd-Ⅰa (M/Z 386) and Pd -Ⅱ(M/Z 426) are found in P.praeruptorum,but not found in P.decursivum.The nodakenetin is found in P. decursivum,but not found in P.praeruptorum.The linear relations are show as follows:Y_Pd-Ⅰa=1.111X_Pd-Ⅰa+0.021(n=6 r=0.9995),ranging from 0.175 to 1.919 μg;Y_Pd-Ⅱ=1.189X_Pd-Ⅱ -0.013(n=6 r=0.9996),ranging from 0.086 to 0.950 μg;Y_Nodakenetin=0.901X_Nodakenetin -0.024 (n=6 r=0.9999),ranging from 0.089 to 1.062 μg.The recoveries for Pd-Ⅰa,Pd -Ⅱ and Nodakenetin are 97.29%,98.81% and 96.34% respectively.The contents of characteristic components are different in the samples derived from different growth areas.The same effective component or marking component,which is contained in both P.praeruptorum and P.decursivum is not found.It is suggested that P.praeruptorum and P.decursivum could be distinguished by GC/MS,but their qualities could not be controlled in the base of the same control component.