| 引用本文： |
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李淑彬,王军,钟英长.多轮复合诱变选育抗真菌抗生素高产菌株及其发酵条件研究[J].广西科学,2003,10(2):135-138. [点击复制]
- Li Shubin,Wang Jun,Zhong Yingchang.Complex Mutagensis and Screening of High Productivity Strains of Antifungal Antibiotic and Their Fermentive Conditions[J].Guangxi Sciences,2003,10(2):135-138. [点击复制]
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| 摘要: |
| 对抗真菌抗生素Terreic acid-179M (简称179M)产生菌黄柄曲霉(Aspergillusf lavipes)进行紫外线、亚硝基胍、紫外线加亚硝基胍多轮复合诱变,以对自身次生代谢产物抗性作为筛选策略,选育到对自身抗生素抗性提高且发酵相对效价提高到383%的高产菌株。菌落形态观察发现在Sabauraud (SBD)培养基上产孢子丰富且形成黄色孢子和黄色菌丝的179M产量较高。179M最佳发酵温度为28℃,培养基最适起始pH值为6。 |
| 关键词: 抗真菌抗生素 诱变 自身次生代谢产物抗性 发酵条件 |
| DOI： |
| 投稿时间：2003-01-15修订日期：2003-02-28 |
| 基金项目:广东省自然科学基金(No.001214)和国家自然科学基金资助项目(No.30270021)。 |
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| Complex Mutagensis and Screening of High Productivity Strains of Antifungal Antibiotic and Their Fermentive Conditions |
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Li Shubin, Wang Jun, Zhong Yingchang
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| (College of Life Science, South China Normal University, Guangzhou, Guangdong, 510631, China) |
| Abstract: |
| Aspergillus flavipes produced by antifungal antibiotic of Terreic acid-179M(designated as 179M),was treated by UV,NG and UV+NG in sequence.The mutated strains were selected by self-secondary metabolites.The microorganism with relative potency of 179M increased to 383% and increasing resistance to 179M were obtained.It was found that the strain 179M has high productivity with abundant yellow spores and hyphas in the medium Sabauraud.The favor temperature to the fermentation of 179M was 28℃ and the favor initial pH value was 6 |
| Key words: antifungal antibiotic mutagensis resistance of self-secondary metabolites fermentive conditions |
