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  • 李兴芳,樊妙姬,蒋艳明,凌云,韦莉莉.相思根瘤菌的抗逆性初步研究[J].广西科学,2003,10(4):312-314,324.    [点击复制]
  • Li Xingfang,Fan Miaoji,Jiang Yanming,Ling Yun,Wei Lili.Preliminary Study on Rhizobia sp.Acacia for Tolerance to Stresses[J].Guangxi Sciences,2003,10(4):312-314,324.   [点击复制]
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相思根瘤菌的抗逆性初步研究
李兴芳, 樊妙姬, 蒋艳明, 凌云, 韦莉莉
0
(广西大学动物科学技术学院, 南宁市大学路100号 530004)
摘要:
对21株相思根瘤菌和2株大豆根瘤菌菌株进行NaCl、(NH4)2SO4、pH值、高温和低温的耐受性实验,其中耐(NH4)2SO4的实验分别进行了相思根瘤菌在含(NH4)2SO4的平板上的耐受性和在浓度为100mol/L、300mol/L、500mol/L、600mol/L的(NH4)2SO4中的结瘤试验。参试菌株均经过回接实验培养和镜检,培养基均为YMA。结果表明,相思根瘤菌均能耐受的盐浓度为0.5%~2.0%,能在37℃和pH值为6~9的条件下生长。在抗生素抗性方面,相思根瘤菌普遍抗氨卞青霉素,而对卡那霉素和链霉素敏感。相思根瘤菌的耐盐、耐高温和耐氨能力强于慢生大豆根瘤菌。
关键词:  相思根瘤菌  耐盐性  生长温度  pH值耐受性  抗生素抗逆性  耐氨性
DOI:
投稿时间:2003-07-14修订日期:2003-08-18
基金项目:
Preliminary Study on Rhizobia sp.Acacia for Tolerance to Stresses
Li Xingfang, Fan Miaoji, Jiang Yanming, Ling Yun, Wei Lili
(College of Animal Science and Technology, Guangxi University, 100 Daxuelu, Nanning, Guangxi, 530004, China)
Abstract:
Twenty one strains of Rhizobium sp. Acacia and two strains of soybean rhizobium were examined for tolerance to the stresses of NaCl,NH4+,acid,alkali,high temperature and low temperature. The results showed that Rhizobium sp. Acacia strains can grow under wide range of temperature and pH. The Rhizobum sp. The tolerances of Acacia strains to NaCl,high temperature and NH4+ are higher than soybean rhizobium and peanut rhizobium strains.
Key words:  Rhizobium sp.Acacia  salt tolerance  growth temperature  pH value tolerance  antibiotic resistance  ammonia tolerance

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