广西科学

引用本文：

申丹,曾杰,周世良,徐大平,白嘉雨.4种石山植物DNA提取方法筛选[J].广西科学,2005,12(4):340-342. [点击复制]
Shen Dan,Zeng Jie,Zhou Shiliang,Xu Daping,Bai Jiayu.Total DNA Extraction from Four Limestone Plants[J].Guangxi Sciences,2005,12(4):340-342. [点击复制]

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4种石山植物DNA提取方法筛选
申丹^1,2, 曾杰¹, 周世良³, 徐大平¹, 白嘉雨¹
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(1.中国林业科学研究院热带林业研究所, 广东广州 510520;2.北京中医药大学药学院北京望京 100102;3.中国科学院植物研究所系统与进化植物学开放实验室, 北京香山 100093)

摘要:

以石山木本植物掌叶木(Handeliodendron bodinieri)、东京桐(Deutzianthus tonkinensis)、伊桐(Itoa orientalis)和肥牛树(Cephalomappa sinensis)为材料,在常用CTAB法的基础上改良,开发出适合这4种木本植物总DNA提取的3种方法,并将提取出的DNA样品进行ISSR分析,确定出提取这4种石山木本植物的总DNA最佳方法,为研究石山木本植物的遗传多样性奠定基础。

关键词: 植物 DNA 提取 ISSR 石山

DOI：

投稿时间：2005-07-05修订日期：2005-08-22

基金项目:中央级科研院所社会公益研究专项资金项目"石质岩溶山地生态综合整治技术研究与示范"(项目编号:2001DIB10067)的部分内容。

Total DNA Extraction from Four Limestone Plants

Shen Dan^1,2, Zeng Jie¹, Zhou Shiliang³, Xu Daping¹, Bai Jiayu¹

(1.Research Institute of Tropical Forestry, Chinese Academy of Forestry, Guangzhou, Guangdong, 510520, China;2.School of Chinese Materia Medica, Beijing Univ. of Chinese Medicine, Wangjing, Beijing, 100102, China;3.Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Science, Xiangshan, Beijing, 100093, China)

Abstract:

Genetic diversity of limestone plants was less reported in China.In this paper the methods were developed on the basis of CATB,to extract total DNA from young leaves of four limestone plant species including two species involved in the Red Book of Chinese Plants(Vol.1) such as Handeliodendron bodinieri and Deutzianthus tonkinensis,and two species with high economic value such as Itoa orientalis and Cephalomappa sinensis.The DNA samples were successfully used in ISSR analysis.The improved methods will be used in studies on genetic diversity of these species in the future.

Key words: plants DNA extraction ISSR limestone

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