广西地不容种质离体保存技术研究

Study on Preservation of Stephanie kwangsiensis H.S.Lo in Vitro

关键词:  广西地不容  离体保存  生长抑制剂  矮壮素  多效唑  脱落酸  青鲜素

Key words:  Stephanie kwangsiensis H.S.Lo  preservation in vitro  plant growth retardant  CCC  PP₃₃₃  ABA  MH

摘要:

以广西地不容(Stephanie kwangsiensis H.S.Lo))试管苗为材料,研究室温(25±2)℃光照2000lx(12h/d)条件下,培养基中不同无机盐水平(MS、1/2MS、1/4MS)、蔗糖浓度(0、20、40、60g/L)和植物生长抑制剂CCC(0.4、0.8、1.2、1.6、2.0、2.5mg/L)、PP₃₃₃(0.2、0.5、1.0、2.0、4.0、8.0mg/L)、ABA(0.2、0.5、1.0、2.0、4.0、8.0mg/L)、MH(0.2、0.5、1.0、2.0、4.0、8.0mg/L)对广西地不容试管苗保存存活率的影响。结果表明,MS添加蔗糖20~40g/L适合作为广西地不容离体保存的基本培养基,附加一定浓度的CCC、PP₃₃₃、ABA和MH能有效抑制试管苗的旺盛生长,长时间保存后的存活率高;ABA易引起试管苗早衰,保存效果不佳。对广西地不容试管苗保存效果较好的两个培养基配方为:MS+CCC0.4~2.5mg/L+蔗糖30g/L和MS+PP₃₃₃0.2~0.5mg/L+蔗糖30g/L,这两个配方可以不继代连续保存360~380d,存活率60%~80%。

Abstract:

The effects of media (MS、1/2MS and 1/4MS), concentration of sucrose and plant retardants (CCC、PP₃₃₃、ABA、MH)on preservation of Stephanie kwangsiensis H.S.Lo in vitro were studied. According to the results, MS medium with sucrose 20~40g/L could be used as basic medium for the preservation of Stephanie kwangsiensis H.S.Lo in vitro.CCC、PP₃₃₃ and MH in certain concentration could retard the growth of tube plantlets which could be preserved for a long time with high survival rate.But tube plantlets tended to senesce prematurely in the mediums with ABA, in which effects on preservation were not very satisfied.The results showed that MS+CCC 0.4~2.5mg/L+sucrose30g/L and MS+PP₃₃₃ 0.2~0.6mg/L+sucrose 30g/L were better than other combinations in preservation of Stephanie kwangsiensis H.S.Lo in vitro.These two formulas could store the tube plantlets up to the (without transfer) 360~380d at 60%~80% survival rate.