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覃晓媚,陆红萍,黄妹春,周征福,韦薇,黄园,陈晓军.HPLC法测定小儿肺热咳喘口服液中连翘苷的含量[J].广西科学,2011,18(4):359-360,368. [点击复制]
- QIN Xiao-mei,LU Hong-ping,HUANG Mei-chun,ZHOU Zheng-fu,WEI Wei,HUANG Yuan,CHEN Xiao-jun.Determination of Phillyrin in Lung-heat Cough Oral Solution for Children by HPLC[J].Guangxi Sciences,2011,18(4):359-360,368. [点击复制]
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| HPLC法测定小儿肺热咳喘口服液中连翘苷的含量 |
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覃晓媚, 陆红萍, 黄妹春, 周征福, 韦薇, 黄园, 陈晓军
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| (广西博科药业有限公司, 广西南宁 530007) |
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| 摘要: |
| 采用C18色谱柱(4.6mm×250mm,5μm),以乙腈-水(25:75)为流动相,流速1.0ml.min-1,检测波长277nm,建立反相高效液相色谱法(HPLC)测定小儿肺热咳喘口服液中的连翘苷含量。结果表明,连翘苷在0.4132~4.132μg范围内与峰面积呈良好线性关系,r=0.9999,平均加样回收率为98.02%,RSD为1.24%(n=6)。本方法分离度好,干扰小,并具有良好的重复性,适用于小儿肺热咳喘口服液中连翘苷的含量测定。 |
| 关键词: 连翘苷 含量 小儿肺热咳喘口服液 高效液相色谱法 |
| DOI： |
| 投稿时间：2011-01-07 |
| 基金项目: |
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| Determination of Phillyrin in Lung-heat Cough Oral Solution for Children by HPLC |
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QIN Xiao-mei, LU Hong-ping, HUANG Mei-chun, ZHOU Zheng-fu, WEI Wei, HUANG Yuan, CHEN Xiao-jun
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| (Guangxi Boke Pharmaceutical CO., LTD. Nanning, Guangxi, 530007, China) |
| Abstract: |
| The HPLC determination method was used to detect the phillyrin in lung-heat cough oral solution for children by C18 (4.6 mm×250mm, 5μm), and acetonitrile and water (25:75) were used as the mobile phase.The flow was set at 1.0ml·min-1, and the detection wavelength was 277nm.The precision and stability were fine in those conditions.The calibration curve was linear over the range of 0.4132~4.132μg for phillyrin (r=0.9999).The average recovery rate (n=6) was 98.02% for phillyrin.The proposed method can be used in the quantitative analysis of phillyrin in lung-heat cough oral solution for children. |
| Key words: phillyrin content lung-heat cough oral solution for children HPLC |
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