冠瘤海鞘克生活性筛选及低极性组分GC-MS分析

Allelopathic Activity Evaluation and GC-MS Analysis of the Low Polar Components from Styela canopus Savigny

关键词:  冠瘤海鞘  卤虫致死活性  甾醇  GC-MS

Key words:  S.canopus  allelopathic effects  sterols  GC-MS

摘要:

为研究冠瘤海鞘(Styela canopus Savigny)的抗卤虫活性部位及有效成分,采用卤虫致死活性筛选模型,对冠瘤海鞘的提取物及甾体粗晶进行了生物活性测定,并采用GC-MS技术对石油醚相进行了成分分析。结果表明:乙醇提取物和各有机相均有明显克生活性,冠瘤海鞘甾体粗晶的克生活性是石油醚相的3.2倍;从冠瘤海鞘石油醚相中鉴定出12个化合物,主要为脂肪类和甾醇类化合物,而且冠瘤海鞘抗卤虫活性成分主要在乙酸乙酯相和石油醚相中,石油醚相中的甾醇类化合物是冠瘤海鞘的克生活性物质之一。

Abstract:

To screen the allelopathic fractions and active constituents of Styela canopus Savigny, the allelopathic effects of ethanolic extract and organic fractions of S.canopus from the South China Sea were evaluated by the model of lethality to brine shrimp Artemia salina, and the low-polar compounds in the petroleum ether fraction were analysed by GC-MS. The results showed that the allelopathic substances were mainly in the ethyl acetate and petroleum ether fraction. And the allelopathic effects of a sterol mixture which were isolated from S.canopus were about three times higher than the effect of petroleum ether fraction. The components in the petroleum ether fraction were analyzed by GC-MS, and 12 compounds were identified for the first time, most of which were 1ipids and sterols. It was proposed that sterols should be the main allelopathic substances in the petroleum ether fraction of S.canopus.