| 引用本文： |
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司梦莹,师德强,戴友芝,陈跃辉.高效木质素降解菌的筛选及降解性能研究[J].广西科学,2015,22(1):48-52,59. [点击复制]
- SI Meng-ying,SHI De-qiang,DAI You-zhi,CHEN Yue-hui.Screening of High Active Lignin-degrading Strains and the Measurement of Their Lignin Degradation[J].Guangxi Sciences,2015,22(1):48-52,59. [点击复制]
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| 摘要: |
| [目的]筛选纯化及鉴定木质素降解菌,对其降解性能进行初步研究。[方法]从某制浆造纸厂的造纸废水污泥中,筛选获得2株具有木质素降解能力的细菌LD-1和LD-2。通过形态观察和16S rRNA序列分析对菌株进行鉴定;采用苯胺蓝平板脱色法及以TC为指标分别定性定量测定2株细菌的木质素降解能力。[结果]LD-1和LD-2分别被鉴定为Sphingobacterium属和Bacillus属的菌株,命名为Sphingobacterium sp.LD-1和Bacillus sp.LD-2。通过苯胺蓝平板脱色实验,发现LD-1和LD-2均具有良好的木质素降解能力。经测定,两株菌种发酵7 d对木质素的降解率分别达到52.23%和50.36%。[结论]成功筛选到2株能高效降解木质素的菌株LD-1和LD-2。 |
| 关键词: 木质素 造纸废水 细菌 生物降解 |
| DOI：10.13656/j.cnki.gxkx.20150126.005 |
| 投稿时间：2014-10-10修订日期：2014-10-20 |
| 基金项目:广西科技开发计划项目(桂科攻14124004-3-4),广西科学院基本科研业务费资助项目(11YJ24SW13)和湖南省教育厅高校科技经费一般项目(14C1085)资助。 |
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| Screening of High Active Lignin-degrading Strains and the Measurement of Their Lignin Degradation |
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SI Meng-ying1, SHI De-qiang2, DAI You-zhi1, CHEN Yue-hui1
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| (1.Key Laboratory of Heavy Metal Pollution Control, College of Hunan Province, Department of Environmental Science and Engineering, College of Chemical Engineering, Xiangtan University, Xiangtan, Hunan, 411105, China;2.Guangxi Academy of Sciences, Nanning, Guangxi, 530007, China) |
| Abstract: |
| [Objective] This study focused on screening, isolating and identifying lignin-degrading strains, and studying on their lignin degradation.[Methods] Two lignin-degrading bacterial strains (LD-1, LD-2) were isolated from the effluent sludge of a pulp paper mill, and identified by morphological observation and 16S rRNA sequence analysis.Their activity of lignin degradation were measured qualitatively and quantitatively by using plate culture medium with Azure-B and TC index, respectively.[Results] LD-1 and LD-2 were characterised as Gram negative small rod and Gram positive rod.Further, 16S rRNA sequencing showed 99% base sequence homology to Sphingobacterium and Bacillus, and the two strains were finally designated as Sphingobacterium sp.LD-1 and Bacillus sp.LD-2, respectively.LD-1 and LD-2 showed potential of lignin degradation by using plate culture medium with Azure-B.Moreover, the lignin degradation by these two bacterial strains reached 52.23% and 50.36%, respectively, within 7 days.[Conclusion] LD-1 and LD-2 showed high activity of lignin degradation. |
| Key words: lignin papermaking wastewater bacteria biodegradation |
