小球藻(Chlorella vulgaris)抗铵品系的紫外诱变选育

UV Mutagenesis and Screening for Ammonium Tolerant Strains of Chlorella vulgar

关键词:  小球藻  紫外诱变  抗高铵  光能转化效率

Key words:  Chlorella vulgaris  UV mutagenesis  high ammonium tolerant  F_v/F_m

摘要:

[目的]获得耐受高铵浓度的优良养殖小球藻(Chlorella vulgaris)品系,可以有效防止其在养殖过程中被轮虫,纤毛虫等污染生物吞食。[方法]首先对小球藻进行紫外线诱变,然后用高浓度NH₄HCO₃作为筛选压力对其进行筛选。[结果]筛选到具有生长优势的小球藻突变株B4和C6,培养12d,在NH₄HCO₃浓度为800mg/L、1 000mg/L、1 200mg/L条件下,B4和C6的生物量比出发藻株分别提高41.18%和9.54%,32.64%和29.41%,37.74%和22.85%;对比出发藻株,B4和C6的最大光能转化效率有显著提高。[结论]突变株B4和C6在高铵培养条件下更能显示生长优势,且它们的生长优势可能来自光合效率的提高。

Abstract:

[Objective] Breeding the new strains of Chlorella vulgaris that have a tolerance to high concentration of ammonium can efficiently avoid being eaten by the contamination animals such as rotifer, ciliate and so on during cultivation.[Methods] The original strain of Chlorella vulgaris was first mutated by exposing to UV light and then was screened by the high concentration of ammonium in media.[Results] Two mutants, B4 and C6, were acquired with better growth rate than the original one.The biomass of strains B4 and C6 after being cultivated for 12 dincreased 41.18% and 9.54%, 32.64% and 29.41%, 37.74% and 22.85%, respectively, under different ammonium concentration, which was 800mg/L, 1 000mg/L, and 1 200mg/L.The F_v/F_m results also proved a significant improvement on their maximum light conversion efficiency.[Conclusion] The mutants B4 and C6 showed growing advantages under higher ammonium concentration, which may be gained from the enhancement of photosynthesis efficiency.