糖苷合成酶及应用的研究进展

Current Progress in Glycosynthase and Its Application

关键词:  糖苷合成酶  糖基化修饰  生物大分子  黄酮  抗生素

Key words:  glycosynthase  glycosylation  macromolecules  flavonoids  antibiotics

摘要:

糖基化修饰不仅能改善生物大分子和化合物的理化性质如水溶性和稳定性,且有可能增强其原生物活性或赋予新活性。目前,提取分离、化学合成和酶法合成是糖基化修饰产物的主要来源,但前两种方法通常存在产物多样性不足、得率低或糖基化位点特异性差等难题。基于糖基转移酶和糖苷合成酶的酶法糖基化修饰技术则能够较好地解决上述两种方法存在的问题,并展现出一定优势。由于糖基转移酶已被多次综述,本文将概述糖苷合成酶的研究进展,为多功能新型糖苷合成酶的开发和应用提供参考和借鉴。

Abstract:

Glycosylation can not only improve the physiochemical properties of biological macromolecules and small-molecule compounds, such as water solubility and stability, but also enhance their original bioactivity or confer a new activity.At present, extraction, chemical synthesis and enzymatic synthesis are the main tools of obtaining glycosylated products.However, the first two methods often show poor product diversity, low yield or poor specificity.The enzymatic glycosylation based on glycosyltransferase and glycosynthase can be a good solution to these obstacles and exhibits a number of advantages. As glycosyltransferase has been reviewed previously, this paper summarizes the recent progress of glycosynthase and provides reference for the development and application of multifunctional glycosynthase.