不同产地两面针果壳挥发油化学成分及其抗肿瘤活性

Chemical Constituents and Antitumor Activities Research of Essential Oil of the Fruit Shell of Zanthoxylum nitidum (Roxb.)DC.From Different Areas

关键词:  两面针  果壳  挥发油  化学成分  抗肿瘤活性

Key words:  Zanthoxylum nitidum (Roxb.)DC.  fruit shell  volatile oil  constituents  antitumor activities

摘要:

[目的]研究不同产地两面针果壳挥发油的化学成分及其抗肿瘤活性,并比较不同产地两面针果壳挥发油的主要化学成分及含量差异,为两面针植物的综合开发利用提供科学依据。[方法]采用水蒸气蒸馏法提取两面针果壳挥发油,应用GC毛细管柱进行分离,质谱检测,面积归一化法测定其相对含量。采用MTT法检测挥发油对宫颈癌细胞(Hela)、乳腺腺癌细胞(MCF-7)、肺腺癌细胞(A549)、胃癌细胞(MGC-803)、结肠癌细胞(COLO-205)的增殖影响。[结果]不同产地两面针果壳挥发油主要成分基本相同,以烯醇类成分为主,其中芳樟醇含量最高,其次为β-蒎烯、β-水芹烯。MTT法提示两面针果壳挥发油可抑制Hela、MCF-7、A549、MGC-803和COLO-205细胞的生长。[结论]不同产地两面针果壳挥发油主要成分基本相同,但含量有差异。两面针果壳挥发油对Hela、MCF-7、A549、MGC-803和COLO-205细胞增殖有抑制作用。

Abstract:

[Objective] To investigate the chemical constituents and the antitumor activities of volatile oil of the fruit shell of Zanthoxylum nitidum (Roxb.)DC.from different areas, and to compare the main chemical constituents and content differences of essential oil of the fruit shell could provide a scientific foundation for the comprehensive development and utilization of this plant.[Methods] The volatile oil was extracted by steam distillation.GC capillary column was used for separation, and the mass spectrometry detection and the normalization method were used to determine the relative content.MTT assay was adopted to evaluate the cytotoxicity on Hela, MCF-7, A549, MGC-803 and COLO-205 cells.[Results] The main chemical constituents of volatile oil of fruit shell of Zanthoxylum nitidum (Roxb.)DC.from different areas were basically the same, with the enol-type constituents being the major components.Among the main components, the content of linalool was the highest, followed by β-phellandrene and β-pinene.MTT assay indicated that the volatile oil could inhibit the growth of Hela, MCF-7, A549, MGC-803 and COLO-205 cells.[Conclusion] The main chemical components of volatile oil of fruit shell of Zanthoxylum nitidum (Roxb.)DC.from different areas were similar, but the contents were different.The volatile oil of the fruit shell could inhibit the proliferation of Hela, MCF-7, A549, MGC-803 and COLO-205 cells.