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秦惠珍,曹其义,宁莞权,唐健民,邹蓉,朱成豪,韦霄,熊忠臣.响应面优化华腺萼木总黄酮和总多糖的超声波辅助提取工艺[J].广西科学,2020,27(4):368-379. [点击复制]
- QIN Huizhen,CAO Qiyi,NING Guanquan,TANG Jianmin,ZOU Rong,ZHU Chenghao,WEI Xiao,XIONG Zhongchen.Optimization of Ultrasonic Assisted Extraction of Total Flavonoids and Total Polysaccharides from Mycetia sinensis by Response Surface Methodology[J].Guangxi Sciences,2020,27(4):368-379. [点击复制]
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| 响应面优化华腺萼木总黄酮和总多糖的超声波辅助提取工艺 |
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秦惠珍1, 曹其义2, 宁莞权2, 唐健民1, 邹蓉1, 朱成豪1, 韦霄1, 熊忠臣1
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| (1.广西壮族自治区中国科学院广西植物研究所, 广西植物功能物质研究与利用重点实验室, 广西桂林 541006;2.百色学院农业与食品工程学院, 广西百色 533000) |
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| 摘要: |
| 为进一步开发天然甜茶资源，以华腺萼木Mycetia sinensis茎叶为原料，超声波辅助水提法提取其中的总黄酮和总多糖，在单因素试验的基础上用响应面优化华腺萼木总黄酮和总多糖的提取工艺。结果发现，对华腺萼木总黄酮提取影响最大的是乙醇浓度，影响最小的是提取时间；对总多糖提取影响最大的是提取时间，影响最小的是提取温度。最终确定华腺萼木总黄酮提取的最佳工艺为超声波功率300 W，料液比1：95，乙醇浓度75%，提取时间50 min，此工艺条件下总黄酮得率为2.36%；总多糖的最佳提取工艺为提取时间180 min，超声波功率250 W，提取温度80℃，此工艺条件下总多糖得率为82.77%。本研究采用响应面法对华腺萼木总黄酮和总多糖提取条件进行了优化，可以为华腺萼木今后的资源开发提供依据。 |
| 关键词: 华腺萼木 总黄酮 总多糖 响应面优化 超声波辅助提取 |
| DOI：10.13656/j.cnki.gxkx.20200924.006 |
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| 基金项目:广西自然科学基金项目（2015GXNSFBAA139089，2017GXNSFBA198011），广西科技基地和人才专项（桂科AD17129022），广西创新驱动项目（桂科-AA17204056-1）和广西植物研究所基本业务费项目（桂植业18013，桂植业18014）资助。 |
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| Optimization of Ultrasonic Assisted Extraction of Total Flavonoids and Total Polysaccharides from Mycetia sinensis by Response Surface Methodology |
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QIN Huizhen1, CAO Qiyi2, NING Guanquan2, TANG Jianmin1, ZOU Rong1, ZHU Chenghao1, WEI Xiao1, XIONG Zhongchen1
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| (1.Guangxi Key Laboratory of Functional Phytochemicals Research and Utilization, Guangxi Institue of Botany, Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences, Guilin, Guangxi, 541006, China;2.College of Agriculture and Food Engineering, Baise University, Baise, Guangxi, 533000, China) |
| Abstract: |
| In order to further develop the natural sweet tea resources,the stems and leaves of Mycetia sinensis were used as the raw material to optimize the extraction technology of total flavonoids and total polysaccharides from M.sinensis by ultrasonic assisted water extraction.On the basis of single factor experiment,the extraction process of total flavonoids and total polysaccharides was optimized by response surface methodology.The results showed that the ethanol concentration had the greatest influence on the extraction of total flavonoids from M.sinensis and the extraction time had the least influence on it.Extraction time had the greatest effect on the extraction of total polysaccharides and the extraction temperature had the least effect.Finally,the optimum extraction technology of M.sinensis total flavonoids was determined as follows:Ultrasonic power 300 W,ratio of material and liquid 1:95,ethanol concentration 75%,ultrasonic time 50 min,and the yield of total flavonoids under this process condition was 2.36%.The optimum extraction technology of M.sinensis total polysaccharide was determined as follows:Ultrasonic time 180 min,ultrasonic power 250 W,temperature 80℃,and the yield of total flavonoids under this process condition was 82.77%.In this study,response surface methodology was used to rationally optimize the extraction conditions of the total flavonoids and total polysaccharides from M.sinensis,which can provide a basis for future resource development of M.sinensis. |
| Key words: Mycetia sinensis total flavonoids total polysaccharides response surface optimization ultrasonic-assisted extraction |
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