不同来源的黄芪多糖产品质量比较研究

Comparative Study on the Quality of Astragalus Polysaccharide from Different Sources

关键词:  黄芪多糖  紫外分光光度法  外观性状  高效液相色谱示差折光检测法  产品质量

Key words:  Astragalus polysaccharide  ultraviolet spectrophotometry  appearance properties  high performance liquid chromatography refractive index detection  quality

摘要:

为比较不同来源黄芪多糖产品的质量差异，应用紫外分光光度法测定黄芪多糖总多糖含量，采用物理方法对黄芪多糖性状进行外观鉴别，再结合高效液相色谱示差折光法测定黄芪多糖中单糖的组成及含量。研究结果显示：7个不同来源（企业）的黄芪多糖产品外观性状、总多糖含量及单糖的组成和含量有较大的差别。总多糖含量为0.143 0—0.963 3 g/g，单糖组成主要为葡萄糖和阿拉伯糖，两者的峰面积比为（1.75—15.76）：1；外观性状质量比较优的产品其总多糖含量为60.0%—75.0%，葡萄糖和阿拉伯糖的峰面积比值为5.28—5.71。该方法操作简便，结果准确可靠，可用于黄芪多糖的品质全面评价，为黄芪多糖产品品质甄别提供参考。

Abstract:

In order to compare the quality differences of Astragalus polysaccharide products from different sources,ultraviolet spectrophotometry was used to determine the total polysaccharide content of Astragalus polysaccharides. Physical methods were used to identify the appearance of Astragalus polysaccharides properties. And the composition and content of monosaccharides in Astragalus polysaccharides were determined by high performance liquid chromatography refractive index detection. There were great differences in total polysaccharides content,appearance characteristics,and composition and content of monosaccharides of Astragalus polysaccharide products from seven different sources. The total polysaccharide content was 0.143 0-0.963 3 g/g. The monosaccharide compositions were mainly glucose and arabinose,and the peak area ratio was (1.75-15.76):1. For products with better appearance quality,the total polysaccharides content was 60.0%-75.0%,and the peak area ratio of glucose and arabinose was 5.28-5.71. The method is easy to operate,accurate and reliable,which can be used for the comprehensive quality evaluation of Astragalus polysaccharides,and provides a reference for the quality screening of Astragalus polysaccharides.