| 引用本文： |
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陈晓倩,刘永宏,黄炳耀.基于机械搅拌式光照发酵罐的紫球藻高产藻红蛋白培养条件优化[J].广西科学,2020,27(5):541-545. [点击复制]
- CHEN Xiaoqian,LIU Yonghong,HUANG Bingyao.Culture Conditions Optimization for High-yield of Phycoerythrin from Porphyridium cruentum Based on a Light Fermentation Tank with Mechanical Agitator[J].Guangxi Sciences,2020,27(5):541-545. [点击复制]
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| 摘要: |
| 本研究以普通机械搅拌式光照发酵罐为培养装置，采用单因素试验法对紫球藻生产藻红蛋白的条件（搅拌速率、光照强度、氮浓度以及NaCl浓度）进行优化。结果表明，发酵培养紫球藻高产藻红蛋白的最佳搅拌速率、光照强度、氮浓度以及NaCl浓度分别为150 r/min、2 000 Lx、8 mmol/L、20‰。本试验优化了搅拌式光照发酵罐培养紫球藻生产藻红蛋白的条件，为紫球藻藻红蛋白的工业化生产提供基础数据及思路。 |
| 关键词: 紫球藻 藻红蛋白 机械搅拌式光照发酵罐 微藻 单因素分析法 条件优化 |
| DOI：10.13656/j.cnki.gxkx.20201214.016 |
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| 基金项目:广西中医药大学博士科研启动基金项目（2018BS042），广西科技计划项目（2018AD19324），广西中医药大学海洋药物研究院团队科研专项（2018ZD005），广西八桂学者专项（05019055）和广西中医药大学岐黄工程高层次人才团队培育项目（2018006）资助。 |
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| Culture Conditions Optimization for High-yield of Phycoerythrin from Porphyridium cruentum Based on a Light Fermentation Tank with Mechanical Agitator |
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CHEN Xiaoqian, LIU Yonghong, HUANG Bingyao
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| (Institute of Marine Drugs, Guangxi University of Chinese Medicine, Nanning, Guangxi, 530200, China) |
| Abstract: |
| In this study,an ordinary light fermentation tank with mechanical agitator was used as the culture device,and the single factor test method was used to optimize the conditions (stirring rate,light intensity,nitrogen concentration and NaCl concentration) for producing phycoerythrin by Porphyridium sp.The results showed that the optimal stirring rate,light intensity,nitrogen concentration and NaCl concentration for high-producing phycoerythrin of Porphyridium sp.were 150 r/min,2 000 Lx,8 mmol/L,20‰,respectively.This experiment optimizes the conditions for culturing Porphyridium sp.to produce phycoerythrin in agitated light fermentation tank.It provides basic data and ideas for the industrial production of Porphyridium sp. |
| Key words: Porphyridium cruentum phycoerythrin light fermentation tank with mechanical agitator microalgae single factor analysis condition optimization |
