2,4,6-三氯苯酚废水处理过程对污泥性能和菌群结构的影响

Effects of 2,4,6-Trichlorophenol Wastewater Treatment on the Sludge Performance and Microbial Community Structure

关键词:  2,4,6-TCP废水  多糖  蛋白质  酶活性  菌群结构

Key words:  2,4,6-TCP wastewater  polysaccharides  proteins  enzymatic activity  microbial community

摘要:

为考察2,4,6-三氯苯酚（2,4,6-TCP）废水处理过程对污泥性能和菌群结构的影响，利用序批式生物反应器（Sequencing Batch Reactor，SBR）处理2,4,6-TCP模拟废水，通过逐步提高进水2,4,6-TCP浓度（10-50 mg/L）的方式进行试验。结果表明，经10 mg/L 2,4,6-TCP驯化的活性污泥可有效降解进水化学需氧量（Chemical Oxygen Demand，COD）和2,4,6-TCP，提高进水2,4,6-TCP浓度基本不影响污泥性能。当处理不同浓度的进水2,4,6-TCP的SBR处于稳定运行阶段末期时，污泥絮体中多糖和蛋白质含量随进水2,4,6-TCP浓度的提高而升高，脱氢酶（Dehydrogenase，DHA）、过氧化氢酶（Catalase，CAT）和超氧化物歧化酶（Superoxide Dismutase，SOD）的活性同样随进水2,4,6-TCP浓度的提高而升高。经2,4,6-TCP驯化的污泥中降解2,4,6-TCP的功能菌属显著富集，虽然不同浓度的进水2,4,6-TCP和不同的SBR运行阶段影响微生物多样性，但不同污泥中的微生物菌属具有一定的相似性。因此，通过逐步提高进水2,4,6-TCP浓度的方式驯化污泥可实现废水中2,4,6-TCP的有效去除。本研究的实施可为氯酚废水及其他难降解工业废水的处理提供科学指导。

Abstract:

In order to investigate the effect of 2,4,6-trichlorophenol (2,4,6-TCP) wastewater treatment process on sludge performance and bacterial community structure, a sequencing batch reactor (SBR) was used to treat 2,4,6-TCP simulated wastewater. The experiment was carried out by gradually increasing the influent 2,4,6-TCP concentration (10-50 mg/L). The results showed that the activated sludge acclimated by 10 mg/L 2,4,6-TCP could effectively degrade the influent chemical oxygen demand (COD) and 2,4,6-TCP, and increasing the concentration of the influent 2,4,6-TCP basically did not affect the sludge performance. After the SBR operated at the end of stable operational stages by treating different concentrations of influent 2,4,6-TCP, the contents of polysaccharides and proteins in sludge flocs were increased with the increase of influent 2,4,6-TCP, and the activities of dehydrogenase (DHA), catalase (CAT) and superoxide dismutase (SOD) were also increased with the increase of influent 2,4,6-TCP. The functional genera for 2,4,6-TCP degradation were significantly enriched after being acclimated by 2,4,6-TCP. Although the microbial diversity was affected by the different concentrations of influent 2,4,6-TCP and different stages of SBR operation, the microbial genera in different sludge had a certain similarity.Therefore, the effective removal of 2,4,6-TCP from wastewater could be achieved by acclimated sludge through gradually increasing the influent 2,4,6-TCP concentration.The implementation of this study could provide the scientific guidance for the treatment of chlorophenols wastewater and other refractory industrial wastewater.