大叶钩藤中柯诺辛碱B及柯诺辛碱的含量测定及分析

Determination and Analysis of the Content of Corynoxine B and Corynoxine in Uncaria macrophylla Wall.

关键词:  大叶钩藤  柯诺辛碱B  柯诺辛碱  含量测定  采收时间  药用部位

Key words:  Uncaria macrophylla Wall.  Corynoxine B  Corynoxine  content determination  harvest time  medicinal parts

摘要:

为建立大叶钩藤Uncaria macrophylla Wall.中柯诺辛碱B （Corynoxine B）及柯诺辛碱 （Corynoxine）的含量测定方法，采用高效液相色谱法测定在不同采收月份大叶钩藤及不同药用部位中柯诺辛碱B及柯诺辛碱的含量。测定条件：Inertsil ODS-3 C₁₈色谱柱（4.6 mm×250 mm，5 μm），流动相为甲醇-0.02%三乙胺（68∶32），流速1 mL/min，检测波长246 nm，柱温25℃。实验结果表明：柯诺辛碱B进样量为0.130-1.950 μg，柯诺辛碱进样量为0.296-4.440 μg，与峰面积呈良好的线性关系，相关系数r均为1.000 0 （n=5）；柯诺辛碱B和柯诺辛碱的平均回收率（n=6）分别为99.47%和100.06%，RSD分别为1.56%和0.98%。本法简便易行，重现性好，可为钩藤药材的质量控制提供科学依据。

Abstract:

In order to establish a method for the determination of Corynoxine B and Corynoxine in Uncaria macrophylla Wall., the contents of Corynoxine B and Corynoxine in different harvest months and different medicinal parts were determined by high performance liquid chromatography. The analysis was carried out on an Inertsil ODS-3 C₁₈ column (4.6 mm×250 mm, 5 μm) using a mixture of 68% methanol and 32% triethylamine aqueous solution with concentration of 0.02% as the mobile phase at a flow rate of 1 mL/min. The column temperature was 25℃ and the wavelength for measurement was 246 nm. The experimental results showed that the sample size of Corynoxine B was 0.130-1.950 μg, and the sample size of Corynoxine was 0.296-4.440 μg. There was a good linear relationship with the peak area, and the correlation coefficient r was both 1.000 0 (n=5). The average recovery (n=6) for Corynoxine B and Corynoxine was 99.47% and 100.06%, respectively.RSD was 1.56% and 0.98% respectively. The method is simple, reproducible and can provide scientific basis for the quality control of Uncaria rhynchophylla.