山口红树林土壤芽孢杆菌多样性及纤维素酶活性筛选研究

Study on Diversity and Screening of Cellulase Activity of Bacillus from Shankou Mangrove Soil

关键词:  广西红树林  土壤  芽孢杆菌  物种多样性  纤维素酶活性

Key words:  mangroves in Guangxi  soil  Bacillus  genetic diversity  cellulase activity

摘要:

为探究广西山口红树林保护区内土壤中芽孢杆菌的多样性，挖掘具有稳定性好、耐高温的纤维素酶活性菌株，本研究利用可培养技术和基于16S rRNA基因序列的系统发育树分析研究红树林土壤中可培养芽孢杆菌的多样性，并以羧甲基纤维素钠为唯一碳源，结合刚果红纤维素培养法、滤纸条崩解试验、纤维素酶活力测定法，对分离出的芽孢杆菌开展纤维素降解活性研究。结果表明，从红树林土壤中共分离出171株芽孢杆菌，隶属于4科12属40种，其中芽孢杆菌属为优势菌属；从中筛选出9株具有显著纤维素酶活性的芽孢杆菌，其中4株具有显著的热稳定性。广西山口红树林自然保护区内土壤中蕴含着十分丰富的可培养芽孢杆菌种类，且部分菌株呈现出显著的纤维素酶活性，具有较大的挖掘潜力。

Abstract:

In order to investigate the diversity of Bacillus in the soil of Shankou mangrove reserve in Guangxi,the strains with good stability and high temperature resistant cellulase activity were excavated.In this study,the diversity of cultivable bacillus in mangrove soil was studied by culturable method and phylogenetic tree analysis based on 16S rRNA gene sequences.Carboxymethyl cellulose sodium was selected as the only carbon source.Combined with Congo red cellulose culture method,filter paper strip disintegration test and cellulose activity assay method,the cellulose degradation activity research on the isolated Bacillus was carried out.The results showed that a total of 171 Bacillus strains were isolated from mangrove soil,belonging to 4 families,12 genera and 40 species,among which Bacillus was the dominant genus.9 Bacillus strains with significant cellulase activity were screened out,and four of them had significant thermal stability.There are abundant cultivable Bacillus species in the soil of Shankou Mangrove Nature Reserve in Guangxi,and some strains show significant cellulase activity,which has great potential for excavation.