| 引用本文： |
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黄岛平,陈秋虹,陈建红,徐慧.高效液相色谱法测定龙眼肉多糖的单糖组成[J].广西科学院学报,2010,26(3):234-236. [点击复制]
- HUANG Dao-ping,CHEN Qiu-hong,CHEN Jian-hong,XU Hui.Analysis of Monosaccharide Composition in Polysacch-aride of Arillus Longan by High Performance Liquid Chromatography[J].Journal of Guangxi Academy of Sciences,2010,26(3):234-236. [点击复制]
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| 摘要: |
| 为了研究分析龙眼肉多糖的单糖组成,采用水提醇沉法提取龙眼肉多糖,用三氟乙酸水解多糖成单糖,经衍生化试剂1-苯基-3-甲基-吡唑啉酮(PMP)衍生化后,利用高效液相色谱法(HPLC)分析单糖的PMP衍生物。结果表明,龙眼肉多糖由甘露糖、鼠李糖、半乳糖醛酸、葡萄糖、半乳糖、木糖、阿拉伯糖7种单糖组成。龙眼肉多糖是一种酸性杂多糖,其中以葡萄糖、阿拉伯糖、半乳糖、半乳糖醛酸为主。 |
| 关键词: 高效液相色谱法 单糖 组成 多糖 龙眼肉 |
| DOI： |
| 投稿时间：2010-04-30 |
| 基金项目:广西科学研究与技术开发计划项目(桂科攻0815009-2-5)资助。 |
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| Analysis of Monosaccharide Composition in Polysacch-aride of Arillus Longan by High Performance Liquid Chromatography |
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HUANG Dao-ping, CHEN Qiu-hong, CHEN Jian-hong, XU Hui
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| (Guangxi Research Center of Analysis and Testing, Nanning, Guangxi, 530022, China) |
| Abstract: |
| The monosaccharide composition of polysaccharides from Arillus Longan was Analyzed.The polysaccharides was extracted by hot distilled water,precipitated by alcohol,and formed monosaccharidedes by TFA hydrdyzation.The hydrolysate reacted with 1-phenyl-3-methyl-5-pyrazolone (PMP) to produce the PMP derivatives that were analyzed by HPLC.The Polysaccharides from Arillus Longan was comprised mannose,rhamnose,galacturonicaicd,glucose,galactose and xylose arabonose.The polysaccharides from Arillus Longan was an acid heteropolysaccharide.Scientific data of polysacaccharides from Arillus Longan for further development and research is provided. |
| Key words: high performance liquid chromatography monosaccharide composition polysaccharides Arillus Longan |
