高效液相色谱法测定野生毛葡萄中的白藜芦醇

Determination of Resveratrol in Wild Grape by HPLC

关键词:  高效液相色谱法  白藜芦醇  野生毛葡萄

Key words:  HPLC  resveratrol  wild grape

摘要:

为了建立整颗野生毛葡萄中白藜芦醇含量的高效液相色谱分析方法,采用组织分散匀质器将葡萄匀浆后用无水硫酸钠研磨干燥均匀样品,再用乙酸乙酯提取作为前处理方法,通过优化各种实验条件,选择色谱柱为SymmetryshielyTM RP18柱(4.6 mm×250mm,5μm),流动相为乙腈-0.1%醋酸溶液(25:75),检测波长为306nm的实验方法进行实验。结果表明,白藜芦醇在1.32~4.80μg/ml的范围内线性关系良好,平均回收率为97.3%,RSD为2.24%。该方法简单准确,重现性好,可以用于山葡萄酒原料野生毛葡萄的质量检测。

Abstract:

A method for determination of resveratrol in the whole wild grape by HPLC was established.The grapes was homogenated by homogeneous dispersion device and the anhydrous sodium sulfate was used to grind and dry the samples.Then the samples were extracted by using ethyl acetate as the pre-treatment methods.By optimizing a variety of experimental conditions,a SymmetryshielyTM RP18(4.6 mm×250mm,5μm) was selected as column, with acetonitrile:0.1%HAC(25:75) as mobile Phase and UV detection wavelength at 306nm.The results demonstrated the calibration curve of resveratrol was linear between 1.32~4.80μg/ml and the average recovery was 97.3% with RSD equating to 2.24%.This method was proved to be simple, rapid, accurate and can be used for the quality determination of wild grape.