| 引用本文： |
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赵翠红,陆巧燕,闫莲姣,黎瑞,刘布鸣.高效液相色谱法测定苯佐卡因含量及其有关物质[J].广西科学院学报,2010,26(3):248-251. [点击复制]
- ZHAO Cui-hong,LU Qiao-yan,YAN Lian-jiao,LI Rui,LIU Bu-ming.Determination of Benzocaine and Its Related Substances by RP HPLC[J].Journal of Guangxi Academy of Sciences,2010,26(3):248-251. [点击复制]
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| 摘要: |
| 采用高效液相色谱法测定苯佐卡因的含量及其有关物质。色谱柱为ODS-BP柱(250mm×4.6mm,5μm),流动相为1.0mol/L冰醋酸溶液-甲醇(60:40),流速1.0 ml/min,检测波长290nm。苯佐卡因在5~125μg/ml浓度范围内线性良好(r=0.9999)。重复性试验RSD为0.78%,苯佐卡因峰与其他峰之间的分离良好。本方法操作简单,测定快速、准确,专属性好,适用于苯佐卡因的含量及有关物质的测定。 |
| 关键词: 高效液相色谱法 苯佐卡因 含量 有关物质 |
| DOI： |
| 投稿时间：2010-06-08 |
| 基金项目: |
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| Determination of Benzocaine and Its Related Substances by RP HPLC |
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ZHAO Cui-hong1, LU Qiao-yan1, YAN Lian-jiao1, LI Rui1, LIU Bu-ming2
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| (1.Nanning Dizhi Pharmaceutical Co. Ltd., Nanning, Guangxi, 530003, China;2.Cuangxi Institute of Traditional Medical and Pharmaceutical Sciences, Nanning, Guangxi, 530022, China) |
| Abstract: |
| Method of high performance liquid chromatography (HPLC) was established for the determination of Benzocaine and its related substances.A ODS-BP column (250mm×4.6mm,5μm) was used.The mobile phase was 1.0mol/L acetic acid-methanol(60:40).The flow rate was 1.0ml/min and the detection wavelength was 290nm.Benzocaine and its related substances were completely separated.The calibration curve of Benzocaine was linear in the range of 5 to 125μg/ml,which correlation cofficient was 0.9999.RSD of the values of repeatability was 0.78%.The method is simple, specific, accurate with good reproducibility,which can be used for the determination of Benzocaine. |
| Key words: HPLC benzocaine quantification related substances |
