超高效液相色谱法测定露酒中的枸橼酸西地那非

Detection of Sildenafil Citrate in the Liqueur by Ultra Performance Liquid Chromatography

关键词:  超高效液相色谱  枸橼酸西地那非  露酒

Key words:  ultra performance liquid chromatography  sildenafil citrate  liqueur

摘要:

为了建立露酒中非法添加枸橼酸西地那非的一种快速、准确、灵敏高的专属性检测方法,采用反相超高效液相色谱法,以C₁₈柱(2.1mm×100mm,1.7μm),柱温30℃,检测波长293.6nm,流动相为乙腈:水:冰醋酸:三乙胺(35:65:0.8:0.4;V/V),流速0.15ml/min的条件进行实验。结果枸橼酸西地那非在0.05~50μg/ml范围内线性关系良好,相关系数r=0.9998,平均回收率为98.93%,RSD=1.86%。建立的方法准确、可靠,可以用于露酒中枸橼酸西地那非的含量检测。

Abstract:

In order to estabish a quick,accurate,sensitive method for analysis of sildenafil citrate,which illegally added into the liqueur,reversed phase ultra performance liquid chromatography was used.The column was C₁₈ 2.1×100mm.Column temperature was 30℃.The mobile phase was acetonitrile:water:acetic acid:triethylamine(35:65:0.8:0.4;V/V) with flow rate 0.15 ml/min and the detection wavelength was 293.6 nm.The linear range of the calibration curve of sildenafil citrate were 0.05~50μg/ml (r=0.9998).The mean rates of recovery was 98.93% and the relative standard deviations (n=6) was 1.86%. The method is accurate, realiable that can be used to determine sildenfil citrate in the liqueur.