白虎头红树林区海泥可培养细菌分离鉴定及其活性研究

Diversity and Bioactive of Cultivated Marine Bacteria Isolated from the Sea Bed Mud at the Mangrove Zone of Baihutou

关键词:  海洋细菌  系统发育分析  抗菌活性  卤虫致死活性

Key words:  marine bacteria  phylogeny  antimicrobial activity  brine shrimp lethality

摘要:

[目的]研究北海白虎头红树林区海泥可培养细菌多样性及其代谢产物的生物活性。[方法]采用纯培养法和分子生物学方法对样品中细菌多样性进行研究,利用滤纸片法和卤虫致死法测试其生物活性。[结果]从海泥中分离获得相关可培养细菌17株,选取9个代表性菌株采用16S rRNA基因序列的系统发育分析后发现,其中5株菌属于Firmicutes门,其余4株菌为Proteobacteria门。5株细菌的代谢产物有较好的抑菌活性,2株菌的代谢产物对卤虫致死活性明显,其中1株菌兼具两种活性。[结论]北海白虎头红树林区海泥中的细菌多样性较为丰富,并且部分细菌具有一定的生物活性。

Abstract:

[Objective] To investigate the diversity and bioactive of culturable bacteria isolated from the sea bed mud at the mangrove zone of Baihutou, Beihai city.[Methods] Cultivated marine bacteria were isolated from the sea bed mud at the mangrove zone of Baihutou by using conventional culture-dependent method, and then investigate their diversity by using phylogenetic analysis based on 16S rRNA gene sequence comparisons. Activities of the crude extract of strains were isolated and tested through filter paper method and Brine shrimp lethality.[Results] 17 bacterial strains were isolated from the sample in media(2216E,NA and ISP 2) supplement with seawater. According to morphological and biochemical characteristics, 9 strains were selected to perform a phyloenetic analysis based on their 16S rRNA gene sequences. Results showed that 9 strains isolated represented 9 species, 5 strains were the Firmicutes(55.6%) and 4 strains were the Proteobacteria (44.4%). The crude extract of 5 strains(BH-7, 9, 11, 13, 14, 15) were shown antibacteria activity, and 2 strains(BH-5, 15) showed obvious brine shrimp lethality. The BH-15 showed both activities.[Conclusion] Cultivated marine bacteria isolated from the sea bed mud at the mangrove zone of Baihutou,Beihai city, showed their potential value to the development of new drugs.