苎麻绿原酸体外抗乙型肝炎病毒的作用研究

Research of Anti-hepatitis B Virus Effects of Chlorogenic Acid Extracted from Ramie in Vitro

关键词:  苎麻  绿原酸  HepG 2 2.2.15细胞  HBsAg  HBeAg

Key words:  ramie  chlorogenic acid  HepG 2 2.2.15 cells  HBsAg  HBeAg

摘要:

为了考察苎麻绿原酸对HepG 2 2.2.15细胞毒性作用，以及对其HBsAg、HBeAg表达的抑制作用，本研究提取苎麻中的绿原酸，配成100.00 μg/mL、50.00 μg/mL、25.00 μg/mL、12.50 μg/mL和6.25 μg/mL 5个浓度，作用于HepG 2 2.2.15细胞，在第3日、第6日和第9日分别收集上清液，四甲基噻唑蓝（MTT）法检测药物的细胞毒性；酶联免疫（ELISA）法检测HBsAg和HBeAg水平。实验结果表明：苎麻绿原酸对HepG 2 2.2.15细胞无毒性作用，其对HBsAg的表达呈现一定的抑制作用，且呈时间依赖性和浓度依赖性抑制，在第9日，最高浓度100.00 μg/mL时抑制率为70.12%；对HBeAg的分泌无抑制作用。苎麻绿原酸无细胞毒性作用，能明显抑制HepG 2 2.2.15细胞HBsAg的表达。

Abstract:

To investigate the cytotoxic effect of chlorogenic acid extracted from ramie on HepG 2 2.2.15 cells and its inhibitory effect on the expression of HBsAg and HBeAg. Chlorogenic acid of ramie was extracted and formulated into different concentration of 100.00 μg/mL, 50.00 μg/mL, 25.00 μg/mL, 12.50 μg/mL and 6.25 μg/mL to act on the HepG 2 2.2.15 cells. Supernatants of the cell cultures were collected on the 3rd, 6th and 9th day. The methyl thiazol tetraztlium (MTT) method was used to evaluate the cytotoxic effects. And ELISA assay was used to test the levels of HBsAg and HBeAg. The results showed that ramie chlorogenic acid had no toxic effect on HepG 2 2.2.15 cells. In ELISA, ramie chlorogenic acid inhibited the expression of HBsAg in a time-dependent and concentration-dependent manner. On the 9th, the inhibition rate was 70.12% at the highest concentration of 100.00μg/mL. However, it had no inhibitory effect on the secretion of HBeAg. The chlorogenic acid extracted from ramie has no cytotoxic effect and can significantly inhibit the expression of HBsAg in HepG 2 2.2.15 cells.