麦芽糖淀粉酶CDS 1-3在淀粉糖化过程中的作用分析

Analysis of the Role of Maltogenic Amylase CDS 1-3 in the Process of Starch Saccharification

关键词:  麦芽糖淀粉酶  木薯淀粉  支链淀粉  支链淀粉水解酶  协同糖化

Key words:  maltogenic amylase  cassava starch  amylopectin  amylopectin hydrolase  synergistic saccharification

摘要:

为探究多糖相互作用对淀粉糖化速率的影响，本研究使用较传统麦芽糖淀粉酶更具支链淀粉水解能力的麦芽糖淀粉酶CDS 1-3，将其与α-淀粉酶及糖化酶协同进行淀粉糖化，并对糖化液进行还原糖测定以及高效液相色谱分析。结果表明：反应到30 min时，CDS 1-3作用效果最明显，相比于Control组，CDS 1-3组糖化液的葡萄糖当量(Dextrose Equivalent,DE)值提高了4.51%。随着反应的进行，DE值提高速率降低。反应到60 min时，DE值提高了1.17%；反应到90 min时，DE值提高了2.12%。高效液相色谱结果显示，在整个反应过程中，CDS 1-3组葡萄糖和麦芽糖的产量始终高于Control组，说明CDS 1-3可有效加快木薯淀粉糖化速率，可应用于淀粉工业中。

Abstract:

To investigate the effect of multi-enzyme interaction on starch saccharification rate,maltogenic amylase CDS 1-3,which was more capable of amylopectin hydrolysis than traditional maltogenic amylase,was combined with α-amylase and glucoamylase for starch saccharification,and the reducing sugar in saccharification liquid was determined and analyzed by high performance liquid chromatography in this study.The results showed that when the reaction was 30 min,the effect of CDS 1-3 was the most obvious.Compared with the Control group,the Dextrose Equivalent (DE) value of the saccharification solution in the CDS 1-3 group was increased by 4.51%.As the reaction proceeded,the rate of increase in DE value decreased.When the reaction was 60 min,the DE value was increased by 1.17%;when the reaction was 90 min,the DE value was increased by 2.12%.The results of high performance liquid chromatography showed that the yields of glucose and maltose in the CDS 1-3 groups were always higher than those in the Control group during the whole reaction process,which indicated that CDS 1-3 could effectively accelerate the saccharification rate of cassava starch and could be used in the starch industry.