银边红雀珊瑚(Pedilanthus tithymaloides(L) Poir.var.cuculatus Hort.)快速繁殖研究

A Study on the Rapid Micropropagation of Pedilanthus tithymaloides (L.) Poir.var.cuculatus Hort

关键词:  银边红雀珊瑚  微型扦插  快速繁殖

Key words:  Pedilanthus tithymaloides(L.) Poir.var.cuculatus Hort.  microcutting  rapid micropropagation

摘要:

顶芽或腋芽在添加6-苄基氨基嘌呤(6-BA)3ppm和萘乙酸(NAA)0.5ppm的MS培养基培养30d,长4~6cm,腋芽3~7个。此时,将它们分切成单芽切段,转到添加6-BA2ppm的MS培养基,继代培养周期21d,繁殖系数5~8。芽长2~3cm时用添加吲哚丁酸(IBA)1ppm的MS培养基进行生根培养,培养21d,生根率87%,移栽成活率93%。

Abstract:

Apical buds or axillary buds were cultured on the MS medium supplemented with benzylaminopurine (6-BA) 3 mg/L and naphthalene-acetic acid (NAA) 0.5 mg/L for 30 d. They grew to be 4~6 cm in length with 3~7 axillary buds. Then, they were cut into segments with one bud,and cultured on MS medium supplemented with 6-BA 2 mg/L for subculture. Subculture cycle was 21 d and multiplication rate was 5~8. Buds were cultured on MS medium supplemented with indolebutyric acid (IBA) 1 mg/L for rooting when length of them was 2~3 cm. Rooting rate was 87% and 93% rooting plantlets were successfully transplanted to pots in the greenhouse.