| 引用本文： |
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雷丹青,李晓飚,舒雨雁.中华眼镜蛇毒神经生长因子的研究[J].广西科学,1997,4(1):48-52,56. [点击复制]
- Lei Danqing,Li Xiaobiao,Shu Yuyan.The Study of Nerve Growth Factor in Chinese Cobra[J].Guangxi Sciences,1997,4(1):48-52,56. [点击复制]
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| 摘要: |
| 采用CM-SepharoseCL-6B和SephosilC8以及HPLC层析方法,自广西产中华眼镜蛇毒中分离纯化神经生长因子(NGF),此NGF经8d鸡胚背根神经节体外培养证明具有促进神经纤维生长的活性。经HPLC及聚丙烯酰胺凝胶电泳鉴定为单一组成,经SDSPAGE及HPLC测定分子量分别为24.1KD和24.9KD,等电聚焦电泳测得pI为8.2,氨基酸组分分析表明NGF含酸性氨基酸较少,通过125|标记NGF测定出NGF在生物体内主要分布于肾、肺、及周围神经。 |
| 关键词: 中华眼镜蛇毒 神经生长因子 电泳 分布 |
| DOI： |
| 投稿时间：1996-08-07修订日期：1996-11-08 |
| 基金项目:广西科委自然科学基金和区教委资助项目。 |
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| The Study of Nerve Growth Factor in Chinese Cobra |
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Lei Danqing, Li Xiaobiao, Shu Yuyan
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| (The Snake Venom Research Institute of Guangxi Medical University, 6 Binhu Road, Nanning, Guangxi, 530021) |
| Abstract: |
| Nerve growth factor (NGF) was purified from the venom of Chinese Cobra (Naja naja atra) by the CM Sepharose CL-6B column chromatography and sephasil C8 reversed-phase chromatography,and then by the HPLC. The purified NGF was shown to be homogeneous in discontinuous PAGE,in SDS-PAGE and in High-pressure Liquid Chromatography (HPLC). In vitro, the greatest outgrowth of the dorsal root ganglias of chicken embryos invoked by NGF at a concentration of 100 ng/mL. The molecular weight was estimated to be about 24.9 KD by HPLC, and to be 24.1 KD by SDS-PAGE. The isoelectric point was about 8.2. Amino acid analysis showed that NGF contained less residues of acidic amino acid.In vivo, the distribution of 125|-NGF was found to be concentrated in kidney, peripheral nerve, liver and lung. |
| Key words: Chinese Cobra (Naja naja atra) venom NGF Electrophoresis distribution |
