支气管哮喘患者吸入白细胞介素-5所致细胞间粘附因子增高的研究

Increased Concentrations of Soluble Intracellular Adhesion Molecule-1 in Bronchial Asthmatics Caused by Inhaled Interleukin-5

关键词:  白细胞介素-5  细胞间粘附因子  支气管哮喘

Key words:  Interleukin-5  intracellular adhesionmolecule-1  pathogenesis asthma

摘要:

采用随机、双盲及设置对照组的办法进行研究。在吸入IL-5之前,对8例不吸烟的哮喘患者及6例非哮喘过敏性疾病患者测定其痰及血清的sICAM-1;吸入IL-5后,于2h、24h、48h及72h取上述标本分别测定其sICAM-1的水平。结果示对照组患者的sICAM-1在整个研究过程中都没有明显的改变。但吸入IL-5后支气管哮喘患者于痰中sICAM-1出现明显的增高,于48h达最高峰,但持续不超过72h。同样的结果亦出现在血清中。研究表明IL-5可能是导致支气管哮喘患者痰及血的sICAM-1增高的原因,对非哮喘过敏疾病患者则无此作用。

Abstract:

In a randomized double-blind, placebo-controlled study design, eight nonsmoking patients with allergic asthma and six non-asthmatic allergic subjects were administered recombinant human interleukin (IL)-5 by nebulization, and the concentrations of soluble (s) ICAM-1 in both induced sputum and serum from each subject were determined before and at 2, 24, 48, 72 h after inhalation, respectively. Our results showed that sICAM-1 levels neither in sputum nor serum within the control group had no change from baseline at any time throughout the study. In allergic asthmatics, vehicle challenge was not able to cause any changes in sICAM 1 levels in both sputum and serum. However, there was an increase in sputum sICAM-1 levels after IL-5 inhalation, which increased with time was significantly greater than baseline value, respectively, reaching a maximum at 48 h, and lasted no more than 72 h.And a similar elevation in serum sICAM-1 levels after IL-5 inhalation was observed in asthmatics. Our results indicated that IL-5 was capable of inducing elevations of both sputum and serum sICAM-1 concentrations in allergic asthmatics, but not in non-asthmatic allergic subjects.