高压静电场对蟆叶秋海棠愈伤组织生长的效应

Effect of High Voltage Electrostatic Field (HVEF) on Callus Growth of Begonia rex

关键词:  蟆叶秋海棠  愈伤组织  生长  静电场

Key words:  Begonia rex  callus  growth  electrostatic field

摘要:

用+1.0kV/cm场强高压静电处理蟆叶秋海棠愈伤组织30min,处理组愈伤组织生长速率达90.7mg/d,比对照组高23%,超氧物歧化酶(SOD)活性、蛋白质及可溶性糖含量分别为179.0u/g·FW、14.4mg/g·FW、49.0mg/g·DW,分别比对照增加10.6%、10.8%、7.7%。过氧化物酶(POD)和吲哚乙酸(IAA)氧化酶活性分别为58.0 O.D470/min·g FW,163.8μg/g·hFW,分别比对照下降12.7%和23.3%。

Abstract:

The callus of Begonia rex with 30 bottles per treatment was treated at the high voltage electrostatic field (HVEF) at +1.0 kV/cm for 30 min. The treated callus were cultured at 28℃ chamber temperature with 10 h of light,1800 lx to 2000 lx of illumination each day for 30 days. The results released that the callus growth in treat group was up to 90.7 mg/d, and 23% higher than the control. The activity of SOD, contents of protein and soluble sugar in the treated group were 179.0 u/g·FW,14.4 mg/g·FW,49.0 mg/g·DW respectively, and 10.6%, 10.8%,7.7% higher than the control respectively. The activities of peroxidase and IAA oxidase were 58.0 O.D470/min·g FW,163.8 μg/g·h FW respectively, and 12.7%, 23.3% respectively lower than the control.