| 引用本文： |
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许明珠,张琴,童潼,于海瑞,董兰芳.方格星虫多糖碱法提取工艺研究[J].广西科学,2014,21(2):164-168. [点击复制]
- XU Ming-zhu,ZHANG Qin,TONG Tong,YU Hai-rui,DONG Lan-fang.Optimization of Alkaline Extraction for Polysaccharide from Sipunculus nudus Linnaeus[J].Guangxi Sciences,2014,21(2):164-168. [点击复制]
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| 摘要: |
| [目的]研究碱法提取方格星虫体壁中水溶性方格星虫多糖的条件及优化。[方法]根据料液比、浸提时间、浸提温度、碱液浓度对多糖得率的影响,并通过正交试验得到方格星虫水溶性多糖浸提工艺优选因素组合。[结果]影响方格星虫多糖得率的因素主次顺序为浸提温度 > 料液比 > 浸提时间 > 碱液浓度;最佳浸提条件为温度50℃,料液比1:6 g/mL,时间4h,碱液(NaOH)的质量分数为8%。[结论]最佳浸提工艺条件下多糖浸提提取率最大为1.81%。 |
| 关键词: 方格星虫 多糖 碱提 优化 |
| DOI： |
| 投稿时间：2013-09-04修订日期：2013-10-11 |
| 基金项目:广西科学研究与技术开发计划(桂科攻11107011-6);广西科学院基本科研业务费项目(12YJ25HYS20)资助。 |
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| Optimization of Alkaline Extraction for Polysaccharide from Sipunculus nudus Linnaeus |
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XU Ming-zhu1, ZHANG Qin1, TONG Tong1, YU Hai-rui2, DONG Lan-fang1
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| (1.Guangxi Institute of Oceanology, Key Laboratory of Marine Biotechnology of Guangxi, Beihai, Guangxi, 536000, China;2.Key Laboratory of Biochemistry and Molecular Biology in Universities of Shandong(Weifang University), College of Biological and Agricultural Engineering, Weifang University, Tianrun Bioengineering R & D Center of Weifang New and High-tech Zone, Weifang, Shandong, 261061, China) |
| Abstract: |
| [Objective] To choose the optimum alkaline extraction process of water-soluble polysaccharide from Sipunculus nudus Linnaeus.[Methods] The orthogonal test design with four factors was used to study the effect of the ratio of material to fluid, extraction time, extraction temperature and alkali concentration on the extracting rate of water-soluble polysaccharides from S.nudus.[Results] The order of the factors that affect the yield of S.nudus polysaccharide is extraction temperature > ratio of material to fluid > extraction time > alkali concentration; the optimum extraction conditions were temperature 50℃, ratio of material to fluid 1:6 g/mL, time 4h and alkali (NaOH) content of 8%.[Conclusion] Under the above conditions, the optimum extraction rate is 1.81%. |
| Key words: Sipunculus nudus polysaccharide alkaline extraction optimizing |
